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From roads to biobanks: Roadkill animals as a valuable source of genetic data

PloS one, 2023-12, Vol.18 (12), p.e0290836-e0290836 [Peer Reviewed Journal]

Copyright: © 2023 Coba-Males et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. ;COPYRIGHT 2023 Public Library of Science ;ISSN: 1932-6203 ;EISSN: 1932-6203 ;DOI: 10.1371/journal.pone.0290836 ;PMID: 38060478

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Title:
From roads to biobanks: Roadkill animals as a valuable source of genetic data
Author: Coba-Males, Manuel Alejandro ; Medrano-Vizcaíno, Pablo ; Enríquez, Sandra ; Brito-Zapata, David ; Martin-Solano, Sarah ; Ocaña-Mayorga, Sofía ; Carrillo-Bilbao, Gabriel Alberto ; Narváez, Wilmer ; Salas, Jaime Antonio ; Arrivillaga-Henríquez, Jazzmín ; González-Suárez, Manuela ; Poveda, Ana
Kalendar, Ruslan
Subjects: Amphibians ; Animals ; Animals, Wild ; Biological diversity ; Biological diversity conservation ; Biological Specimen Banks ; Birds ; Comparative analysis ; DNA ; Environmental sciences & ecology ; Genetic aspects ; Health aspects ; Humans ; Identification and classification ; Life sciences ; Liver ; Mammals ; Multidisciplinary ; Roadkills ; Sciences de l’environnement & écologie ; Sciences du vivant ; Vertebrates
Is Part Of: PloS one, 2023-12, Vol.18 (12), p.e0290836-e0290836
Description: To protect biodiversity we must understand its structure and composition including the bacteria and microparasites associated with wildlife, which may pose risks to human health. However, acquiring this knowledge often presents challenges, particularly in areas of high biodiversity where there are many undescribed and poorly studied species and funding resources can be limited. A solution to fill this knowledge gap is sampling roadkill (animals that die on roads as a result of collisions with circulating vehicles). These specimens can help characterize local wildlife and their associated parasites with fewer ethical and logistical challenges compared to traditional specimen collection. Here we test this approach by analyzing 817 tissue samples obtained from 590 roadkill vertebrate specimens (Amphibia, Reptilia, Aves and Mammalia) collected in roads within the Tropical Andes of Ecuador. First, we tested if the quantity and quality of recovered DNA varied across roadkill specimens collected at different times since death, exploring if decomposition affected the potential to identify vertebrate species and associated microorganisms. Second, we compared DNA stability across taxa and tissues to identify potential limitations and offer recommendations for future work. Finally, we illustrate how these samples can aid in taxonomic identification and parasite detection. Our study shows that sampling roadkill can help study biodiversity. DNA was recovered and amplified (allowing species identification and parasite detection) from roadkill even 120 hours after death, although risk of degradation increased overtime. DNA was extracted from all vertebrate classes but in smaller quantities and with lower quality from amphibians. We recommend sampling liver if possible as it produced the highest amounts of DNA (muscle produced the lowest). Additional testing of this approach in areas with different environmental and traffic conditions is needed, but our results show that sampling roadkill specimens can help detect and potentially monitor biodiversity and could be a valuable approach to create biobanks and preserve genetic data.
Publisher: United States: Public Library of Science
Language: English
Identifier: ISSN: 1932-6203
EISSN: 1932-6203
DOI: 10.1371/journal.pone.0290836
PMID: 38060478
Source: GFMER Free Medical Journals
MEDLINE
PubMed Central
Public Library of Science (PLoS)
ProQuest Central
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From roads to biobanks: Roadkill animals as a valuable source of genetic data

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