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Nano-fluorescent quantum dots as substrates for determination of ribavirin in pharmaceuticals and human plasma as well as monitoring of its kinetic interaction with salmon sperm DNA

Journal of analytical science and technology, 2023-12, Vol.14 (1), p.26-16, Article 26 [Peer Reviewed Journal]

The Author(s) 2023 ;The Author(s) 2023. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. ;ISSN: 2093-3371 ;ISSN: 2093-3134 ;EISSN: 2093-3371 ;DOI: 10.1186/s40543-023-00391-4

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Title:
Nano-fluorescent quantum dots as substrates for determination of ribavirin in pharmaceuticals and human plasma as well as monitoring of its kinetic interaction with salmon sperm DNA
Author: Abdel Hakiem, Ahmed Faried ; Boushra, John M. ; Noureldeen, Deena A. M. ; Lashien, Adel S. ; Attia, Tamer Z.
Subjects: Analytical Chemistry ; Binding ; Binding interaction kinetics to ssDNA ; Biomolecules ; Blood plasma ; Carbon ; Carbon dots ; Characterization and Evaluation of Materials ; Chemical synthesis ; Chemistry ; Chemistry and Materials Science ; Commercial capsules and spiked human plasma ; Deoxyribonucleic acid ; Determination of ribavirin ; DNA ; Flourescence spectroscopy ; Fluorescence ; Fluorescence spectroscopy ; Microorganisms ; Monitoring ; Monitoring/Environmental Analysis ; Phosphorus ; Phosphorus and nitrogen co-doped carbon quantum dots ; Quantum dots ; Quenching ; Rate constants ; Research Article ; Ribavirin ; Salmon ; Spectrum analysis ; Sperm ; Substrates ; Turning off (quenching) and turning on (peeling off)
Is Part Of: Journal of analytical science and technology, 2023-12, Vol.14 (1), p.26-16, Article 26
Description: Ribavirin (RIB) was successfully determined by fluorescence spectroscopy upon its quenching to environment friendly phosphorus and nitrogen co-doped carbon quantum dots (PNQDs). Different analytical parameters affecting the fluorescence spectra have been optimized and validated in accordance to the ICH guidelines. The proposed method has provided an efficient tracing of the interaction between RIB molecules and the synthesized QDs in an acidic medium (off-mode). The RIB molecules have shown excellent sensitivity by quenching of the emission band at 401 nm upon excitation at 245 nm throughout a linear range of 0.06–10.00 µg/mL with detection and quantitation limits down to 14.00 and 40.00 ng/mL, respectively. The quenching mode was proven to be static in raw samples and samples extracted of spiked plasma for quenching rate constants of 1.30 × 10 12 L M −1 S −1 and 1.73 × 10 12 L M −1 S −1 , respectively. The proposed method has been successfully applied for determination of RIB in the commercial capsules and spiked human plasma samples with good recovery percentages in between 102.00 and 103.00%. Interestingly, these carbon dots have been utilized as nano-fluorescent platforms for assessment of the binding interaction kinetics between the RIB molecules and salmon sperm DNA (ssDNA). This has been implemented through peeling off the RIB molecules from surface of the PNQDs upon successive addition of the ssDNA and hence fluorescence restoration (turning on). Consequently, this provides a successful monitoring of its antimicrobial potency. It was evidenced a strong binding interaction with a binding constant of 2.38 × 10 4 mol −1 /L. Significantly, this could open doors for an extended application for on-site monitoring of RIB as well as its interactions with biomolecules and microorganisms.
Publisher: Singapore: Springer Nature Singapore
Language: English
Identifier: ISSN: 2093-3371
ISSN: 2093-3134
EISSN: 2093-3371
DOI: 10.1186/s40543-023-00391-4
Source: Geneva Foundation Free Medical Journals at publisher websites
Coronavirus Research Database
ProQuest Central
DOAJ Directory of Open Access Journals
Springer Nature OA Free Journals

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Nano-fluorescent quantum dots as substrates for determination of ribavirin in pharmaceuticals and human plasma as well as monitoring of its kinetic interaction with salmon sperm DNA

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