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Molecular Clocks

Proceedings of the National Academy of Sciences - PNAS, 2001-01, Vol.98 (3), p.944-949 [Peer Reviewed Journal]

Copyright 1993-2001 National Academy of Sciences of the United States of America ;Copyright National Academy of Sciences Jan 30, 2001 ;Copyright © 2001, The National Academy of Sciences 2001 ;ISSN: 0027-8424 ;EISSN: 1091-6490 ;DOI: 10.1073/pnas.98.3.944 ;PMID: 11158575

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Title:
Molecular Clocks
Author: Robinson, Noah E. ; Robinson, Arthur B.
Subjects: Amides ; Amino Acid Sequence ; Animals ; Average linear density ; Biochemistry ; Biological Clocks ; Biological Sciences ; Cytochromes ; Fructose-Bisphosphate Aldolase - chemistry ; Fructose-Bisphosphate Aldolase - metabolism ; Imides ; Indicators and Reagents ; Kinetics ; Mass spectrometers ; Mass Spectrometry ; Mass spectroscopy ; Models, Biological ; Molecules ; Muscle, Skeletal - enzymology ; Oligopeptides - chemical synthesis ; Oligopeptides - chemistry ; Peptide Fragments - chemical synthesis ; Peptide Fragments - chemistry ; Peptide Library ; Peptides ; Proteins ; Rabbits ; Resins ; Scientific imaging
Is Part Of: Proceedings of the National Academy of Sciences - PNAS, 2001-01, Vol.98 (3), p.944-949
Description: A convenient and precise mass spectrometric method for measurement of the deamidation rates of glutaminyl and asparaginyl residues in peptides and proteins has been developed; the rates of deamidation of 306 asparaginyl sequences in model peptides at pH 7.4, 37.0°C, 0.15 M Tris·HCl buffer have been determined; a library of 913 amide-containing peptides for use by other investigators in similar studies has been established; and, by means of simultaneous deamidation rate measurements of rabbit muscle aldolase and appropriate model peptides in the same solutions, the use of this method for quantitative measurement of the relative effects of primary, secondary, tertiary, and quaternary protein structure on deamidation rates has been demonstrated. The measured rates are discussed with respect to the hypothesis that glutaminyl and asparaginyl residues serve, through deamidation, as molecular timers of biological events.
Publisher: United States: National Academy of Sciences
Language: English
Identifier: ISSN: 0027-8424
EISSN: 1091-6490
DOI: 10.1073/pnas.98.3.944
PMID: 11158575
Source: GFMER Free Medical Journals
MEDLINE
PubMed Central

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Molecular Clocks

Copyright 1993-2001 National Academy of Sciences of the United States of America ;Copyright National Academy of Sciences Jan 30, 2001 ;Copyright © 2001, The National Academy of Sciences 2001 ;ISSN: 0027-8424 ;EISSN: 1091-6490 ;DOI: 10.1073/pnas.98.3.944 ;PMID: 11158575

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