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The Elasticity of an Individual Fibrin Fiber in a Clot

Proceedings of the National Academy of Sciences - PNAS, 2005-06, Vol.102 (26), p.9133-9137 [Peer Reviewed Journal]

Copyright 1993/2005 The National Academy of Sciences of the United States of America ;Copyright National Academy of Sciences Jun 28, 2005 ;Copyright © 2005, The National Academy of Sciences 2005 ;ISSN: 0027-8424 ;EISSN: 1091-6490 ;DOI: 10.1073/pnas.0504120102 ;PMID: 15967976

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Title:
The Elasticity of an Individual Fibrin Fiber in a Clot
Author: Collet, Jean-Philippe ; Shuman, Henry ; Ledger, Robert E. ; Lee, Seungtaek ; Weisel, John W. ; Lorand, Laszlo
Subjects: Biochemistry ; Biochemistry - methods ; Biological Sciences ; Blood clots ; Blood Coagulation ; Elastic tissue ; Elasticity ; Enzymes ; Factor XIIIa - chemistry ; Ferries ; Fibrin - chemistry ; Fibrinogen - chemistry ; Humans ; Lasers ; Ligation ; Macromolecular Substances ; Mechanical properties ; Microscopy, Atomic Force ; Microscopy, Confocal ; Moduli of elasticity ; Plasma - metabolism ; Polymers ; Polymers - chemistry ; Stiffness ; Thrombin - chemistry ; Time Factors ; Transglutaminases - chemistry ; Tweezers
Is Part Of: Proceedings of the National Academy of Sciences - PNAS, 2005-06, Vol.102 (26), p.9133-9137
Description: A blood clot needs to have the right degree of stiffness and plasticity to stem the flow of blood and yet be digestable by lytic enzymes so as not to form a thrombus, causing heart attacks, strokes, or pulmonary emboli, but the origin of these mechanical properties is unknown. Clots are made up of a three-dimensional network of fibrin fibers stabilized through ligation with a transglutaminase, factor XIIIa. We developed methods to measure the elastic moduli of individual fibrin fibers in fibrin clots with or without ligation, using optical tweezers for trapping beads attached to the fibers that functioned as handles to flex or stretch a fiber. Here, we report direct measurements of the microscopic mechanical properties of such a polymer. Fibers were much stiffer for stretching than for flexion, as expected from their diameter and length. Elastic moduli for individual fibers in plasma clots were 1.7 ± 1.3 and 14.5 ± 3.5 MPa for unligated and ligated fibers, respectively. Similar values were obtained by other independent methods, including analysis of measurements of fluctuations in bead force as a result of Brownian motion. These results provide a basis for understanding the origin of clot elasticity.
Publisher: United States: National Academy of Sciences
Language: English
Identifier: ISSN: 0027-8424
EISSN: 1091-6490
DOI: 10.1073/pnas.0504120102
PMID: 15967976
Source: Geneva Foundation Free Medical Journals at publisher websites
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PubMed Central

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The Elasticity of an Individual Fibrin Fiber in a Clot

Copyright 1993/2005 The National Academy of Sciences of the United States of America ;Copyright National Academy of Sciences Jun 28, 2005 ;Copyright © 2005, The National Academy of Sciences 2005 ;ISSN: 0027-8424 ;EISSN: 1091-6490 ;DOI: 10.1073/pnas.0504120102 ;PMID: 15967976

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