skip to main content
Language:
Search Limited to: Search Limited to: Resource type Show Results with: Show Results with: Search type Index

Identification and Characterization of Two Novel Geminiviruses Associated with Paper Mulberry ( Broussonetia papyrifera ) Leaf Curl Disease

Plant disease, 2020-11, Vol.104 (11), p.3010-3018 [Peer Reviewed Journal]

ISSN: 0191-2917 ;EISSN: 1943-7692 ;DOI: 10.1094/PDIS-12-19-2597-RE

Full text available

Citations Cited by
  • Title:
    Identification and Characterization of Two Novel Geminiviruses Associated with Paper Mulberry ( Broussonetia papyrifera ) Leaf Curl Disease
  • Author: Qiu, Yuanjian ; Zhang, Song ; Yu, Haodong ; Xuan, Zhiyou ; Yang, Liu ; Zhan, Binhui ; Murilo Zerbini, F. ; Cao, Mengji
  • Is Part Of: Plant disease, 2020-11, Vol.104 (11), p.3010-3018
  • Description: Paper mulberry (Broussonetia papyrifera) is a perennial woody plant used as source material for Cai Lun paper making, in traditional Chinese medicine, and as livestock feed. To identify the presence of viruses in paper mulberry plants affected by a disease with leaf curl symptoms, high-throughput sequencing of total RNA was performed. Analysis of transcriptome libraries allowed the reconstruction of two geminivirus-like genomes. Rolling-circle amplification and PCR with back-to-back primers confirmed the presence of two geminiviruses with monopartite genomes in these plants, with the names paper mulberry leaf curl virus 1 and 2 (PMLCV-1 and PMLCV-2) proposed. The genomes of PMLCV-1 (3,056 nt) and PMLCV-2 (3,757 to 3,763 nt) encode six proteins, with the V4 protein of PMLCV-1 and the V3 proteins of both viruses having low similarities to any known protein in databases. Alternative splicing of an intron, akin to that of mastre-, becurto-, capula-, and grabloviruses, was identified by small RNA (sRNA)-seq and RNA-seq reads mapping to PMLCV-1 and PMLCV-2 antisense transcripts. Phylogenetic analyses and pairwise comparisons showed that PMLCV-1 and PMLCV-2 are most closely related to, but distinct from, two unassigned geminiviruses, citrus chlorotic dwarf associated virus and mulberry mosaic dwarf associated virus, suggesting that they are two new members of the family Geminiviridae. Field investigation confirmed the close association of the two viruses with leaf curl symptoms in paper mulberry plants and that coinfection can aggravate the symptoms.
  • Language: English
  • Identifier: ISSN: 0191-2917
    EISSN: 1943-7692
    DOI: 10.1094/PDIS-12-19-2597-RE
  • Source: Alma/SFX Local Collection
Back to results list
INSPIRE LIBRARY - TON DUC THANG UNIVERSITY (84-028) 37 755 057 Feedback
19 Nguyen Huu Tho St. Dist.7, HCM thuvien@tdtu.edu.vn

Copyright © 2017 INSPIRE LIBRARY - TON DUC THANG UNIVERSITY

Searching Remote Databases, Please Wait