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Characterization of recombinant Bacillus halodurans CM1 xylanase produced by Pichia pastoris KM71 and its potential application in bleaching process of bagasse pulp

Indonesian journal of biotechnology, 2021-03, Vol.26 (1), p.15-24 [Peer Reviewed Journal]

ISSN: 0853-8654 ;EISSN: 2089-2241 ;DOI: 10.22146/ijbiotech.57701

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  • Title:
    Characterization of recombinant Bacillus halodurans CM1 xylanase produced by Pichia pastoris KM71 and its potential application in bleaching process of bagasse pulp
  • Author: Haniyya, Haniyya ; Mulyawati, Lina ; Helianti, Is ; Pinmanee, Phitsanu ; Kocharin, Kanokarn ; Cantasingh, Duriya ; Nimchua, Thidarat
  • Subjects: bacillus halodurans ; biobleaching ; cellulase‐free xylanase ; pichia pastoris ; thermoalkalophilic xylanase
  • Is Part Of: Indonesian journal of biotechnology, 2021-03, Vol.26 (1), p.15-24
  • Description: Thermoalkalophilic xylanases promise potential application in pulp biobleaching to reduce the use of toxic chlorinated chemical agents, which are harmful to the environment. In this study, a thermoalkalophilic endoxylanase gene (bhxyn3) originating from Indonesian indigenous Bacillus halodurans CM1 was cloned into yeast expression vector pPICZα A and expressed in Pichia pastoris KM71 under the control of AOX1 promoter. Recombinant P. pastoris expressed the highest final level of xylanase (146 U/mL) on BMGY medium after five days of cultivation. Optimization of xylanase production on a small scale was carried out by varying the methanol concentrations and the optimal xylanase production by the recombinant P. pastoris was observed in the culture with 2% (v/v) methanol after four days of the induction phase. The recombinant xylanase (BHxyn3E) was thermotolerant and alkalophilic, with an optimal temperature at around 55‐65 °C and under pH 8.0. The enzyme activity was slightly induced by K+, Fe2+, and MoO42‐. Enzymatic bleaching of bagasse pulp with no prior pH adjustment (pH 9) using BHxyn3E at 200 U/g oven dried pulp increased the lightness index (L*) and changed substantially the color a index (a*); however, the treatments did not change the whiteness index in a significant way. Therefore, further optimization and assessment such as adjustment of incubation temperature and pH in biobleaching were needed to reduce the use of harmful chemical agents in industrial applications.
  • Publisher: Universitas Gadjah Mada, Yogyakarta
  • Language: English
  • Identifier: ISSN: 0853-8654
    EISSN: 2089-2241
    DOI: 10.22146/ijbiotech.57701
  • Source: DOAJ Directory of Open Access Journals
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