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Liquid biopsies for omics-based analysis in sentinel mussels
PloS one, 2019-10, Vol.14
[Peer Reviewed Journal]
Distributed under a Creative Commons Attribution 4.0 International License ;ISSN: 1932-6203 ;EISSN: 1932-6203 ;DOI: 10.1371/journal.pone.0223525
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Title:
Liquid biopsies for omics-based analysis in sentinel mussels
Author:
Caza, France
;
Joly de Boissel, Philippine Granger
;
Villemur, Richard
;
Betoulle, Stéphane
;
St-Pierre, Yves
Subjects:
Environmental Sciences
;
Life Sciences
Is Part Of:
PloS one, 2019-10, Vol.14
Description:
Liquid biopsy of plasma is a simple and non-invasive technology that holds great promise in biomedical research. It is based on the analysis of nucleic acid-based biomarkers with pre-dictive potential. In the present work, we have combined this concept with the FTA technology for sentinel mussels. We found that hemocytes collected from liquid biopsies can be readily fixed on FTA cards and used for long-term transcriptome analysis. We also showed that liquid biopsy is easily adaptable for metagenomic analysis of bacterial profiles of mussels. We finally provide evidence that liquid biopsies contained circulating cell-free DNA (ccfDNA) which can be used as an easily accessible genomic reservoir. Sampling of FTA-fixed circulating nucleic acids is stable at room temperature and does not necessitate a cold-chain protection. It showed comparable performance to frozen samples and is ideally adapted for sampling in remote areas, most notably in polar regions threatened by anthropo-genic activities. From an ethical point of view, this minimally-invasive and non-lethal approach further reduces incidental mortality associated with conventional tissue sampling. This liquid biopsy-based approach should thus facilitate biobanking activities and development of omics-based biomarkers in mussels to assess the quality of aquatic ecosystems.
Publisher:
Public Library of Science
Language:
English
Identifier:
ISSN: 1932-6203
EISSN: 1932-6203
DOI: 10.1371/journal.pone.0223525
Source:
Hyper Article en Ligne (HAL) (Open Access)
GFMER Free Medical Journals
PubMed Central
Public Library of Science (PLoS)
ProQuest Central
DOAJ Directory of Open Access Journals
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