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Investigation of lignin deposition on cellulose during hydrothermal pretreatment, its effect on cellulose hydrolysis, and underlying mechanisms

Biotechnology and bioengineering, 2014-03, Vol.111 (3), p.485-492 [Peer Reviewed Journal]

2013 Wiley Periodicals, Inc. ;ISSN: 0006-3592 ;EISSN: 1097-0290 ;DOI: 10.1002/bit.25108 ;PMID: 24037461 ;CODEN: BIBIAU

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Title:
Investigation of lignin deposition on cellulose during hydrothermal pretreatment, its effect on cellulose hydrolysis, and underlying mechanisms
Author: Li, Hongjia ; Pu, Yunqiao ; Kumar, Rajeev ; Ragauskas, Arthur J. ; Wyman, Charles E.
Subjects: Biomass ; Cellulases - metabolism ; Cellulose ; Cellulose - chemistry ; Cellulose - metabolism ; Cellulose - radiation effects ; Cellulose - ultrastructure ; deposition ; enzymatic hydrolysis ; Enzymes ; Hot Temperature ; Hydrolysis ; hydrothermal pretreatment ; inhibition mechanisms ; Lignin ; Lignin - chemistry ; Lignin - metabolism ; Lignin - radiation effects ; Lignin - ultrastructure ; lignin droplets ; Magnetic Resonance Spectroscopy ; Microscopy, Electron, Scanning ; Polymers ; Populus ; Scanning electron microscopy ; Wood
Is Part Of: Biotechnology and bioengineering, 2014-03, Vol.111 (3), p.485-492
Description: ABSTRACT In dilute acid pretreatment of lignocellulosic biomass, lignin has been shown to form droplets that deposit on the cellulose surface and retard enzymatic digestion of cellulose (Donohoe et al., 2008; Selig et al., 2007). However, studies of this nature are limited for hydrothermal pretreatment, with the result that the corresponding mechanisms that inhibit cellulosic enzymes are not well understood. In this study, scanning electron microscope (SEM) and wet chemical analysis of solids formed by hydrothermal pretreatment of a mixture of Avicel cellulose and poplar wood showed that lignin droplets from poplar wood relocated onto the Avicel surface. In addition, nuclear magnetic resonance (NMR) showed higher S/G ratios in deposited lignin than the initial lignin in poplar wood. Furthermore, the lignin droplets deposited on Avicel significantly impeded cellulose hydrolysis. A series of tests confirmed that blockage of the cellulose surface by lignin droplets was the main cause of cellulase inhibition. The results give new insights into the fate of lignin in hydrothermal pretreatment and its effects on enzymatic hydrolysis. Biotechnol. Bioeng. 2014;111: 485–492. © 2013 Wiley Periodicals, Inc. In this study, lignin droplets from poplar wood relocated onto the Avicel surface during hydrothermal pretreatment and significantly impeded cellulose hydrolysis. NMR results showed higher S/G ratios in deposited lignin than the initial lignin in poplar wood. Blockage of the cellulose surface by lignin droplets was confirmed as the main cause of cellulase inhibition. The results give new insights into the fate of lignin in hydrothermal pretreatment and its effects on enzymatic hydrolysis.
Publisher: United States: Blackwell Publishing Ltd
Language: English
Identifier: ISSN: 0006-3592
EISSN: 1097-0290
DOI: 10.1002/bit.25108
PMID: 24037461
CODEN: BIBIAU
Source: MEDLINE
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Investigation of lignin deposition on cellulose during hydrothermal pretreatment, its effect on cellulose hydrolysis, and underlying mechanisms

2013 Wiley Periodicals, Inc. ;ISSN: 0006-3592 ;EISSN: 1097-0290 ;DOI: 10.1002/bit.25108 ;PMID: 24037461 ;CODEN: BIBIAU

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