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Enhancing plant growth and fiber production by silencing GA 2-oxidase

Plant biotechnology journal, 2010-05, Vol.8 (4), p.425-435 [Peer Reviewed Journal]

2010 The Authors. Journal compilation © 2010 Blackwell Publishing Ltd ;2015 INIST-CNRS ;ISSN: 1467-7644 ;EISSN: 1467-7652 ;DOI: 10.1111/j.1467-7652.2009.00480.x ;PMID: 20070875

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Title:
Enhancing plant growth and fiber production by silencing GA 2-oxidase
Author: Dayan, Jonathan ; Schwarzkopf, Maayan ; Avni, Adi ; Aloni, Roni
Subjects: Amino Acid Sequence ; Arabidopsis - enzymology ; Arabidopsis - genetics ; Biological and medical sciences ; biomass ; Biotechnology ; Crosses, Genetic ; fibers ; Fundamental and applied biological sciences. Psychology ; GA 2-oxidase ; Gene Silencing ; Genes, Plant - genetics ; gibberellin ; gibberellins ; Mixed Function Oxygenases - chemistry ; Mixed Function Oxygenases - genetics ; Mixed Function Oxygenases - metabolism ; Molecular Sequence Data ; Nicotiana - cytology ; Nicotiana - enzymology ; Nicotiana - genetics ; Nicotiana - growth & development ; Phenotype ; Plant Shoots - growth & development ; Plant Stems - anatomy & histology ; Plant Stems - cytology ; Plants, Genetically Modified ; Transformation, Genetic ; wood formation ; xylem ; Xylem - growth & development
Is Part Of: Plant biotechnology journal, 2010-05, Vol.8 (4), p.425-435
Description: Enhancing plant height and growth rates is a principal objective of the fiber, pulp, wood and biomass product industries. Many biotechnological systems have been established to advance that task with emphasis on increasing the concentration of the plant hormone gibberellin, or on its signalling. In this respect, the most studied gibberellin biosynthesis enzyme is the GA 20-oxidase which catalyses the rate limiting step of the pathway. Overexpression of the gene resulted in an excessively high activity of the gibberellin deactivating enzyme, GA 2-oxidase. Consequently, this feedback regulation limits the intended outcome. We assume that silencing GA 2-oxidase transcription would abolish this antithetical effect, thereby allowing greater gibberellin accumulation. Here, we show that silencing the gibberellin deactivating enzyme in tobacco model plants results in a dramatic improvement of their growth characteristics, compared with the wild type and GA 20-oxidase over-expressing plants. Moreover, the number of xylem fiber cells in the silenced lines exceeded that of GA 20-oxidase over-expressing plants, potentially, making GA 2-oxidase silencing more profitable for the wood and fiber industries. Interestingly, crossing GA 20-oxidase over-expressing plants with GA 2-oxidase silenced plants did not yield consequential additive effects. Our findings unveil the benefits of silencing GA 2-oxidase to substantially increase tobacco growth and fiber production, which suggest using this approach in cultivated forest plantations and industrial herbaceous plants, worldwide.
Publisher: Oxford, UK: Oxford, UK : Blackwell Publishing Ltd
Language: English
Identifier: ISSN: 1467-7644
EISSN: 1467-7652
DOI: 10.1111/j.1467-7652.2009.00480.x
PMID: 20070875
Source: Wiley Online Library Open Access

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Enhancing plant growth and fiber production by silencing GA 2-oxidase

2010 The Authors. Journal compilation © 2010 Blackwell Publishing Ltd ;2015 INIST-CNRS ;ISSN: 1467-7644 ;EISSN: 1467-7652 ;DOI: 10.1111/j.1467-7652.2009.00480.x ;PMID: 20070875

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