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Strongly enhanced bacterial bioluminescence with the ilux operon for single-cell imaging

Proceedings of the National Academy of Sciences - PNAS, 2018-01, Vol.115 (5), p.962-967 [Peer Reviewed Journal]

Volumes 1–89 and 106–114, copyright as a collective work only; author(s) retains copyright to individual articles ;Copyright © 2018 the Author(s). Published by PNAS. ;Copyright National Academy of Sciences Jan 30, 2018 ;Copyright © 2018 the Author(s). Published by PNAS. 2018 ;ISSN: 0027-8424 ;EISSN: 1091-6490 ;DOI: 10.1073/pnas.1715946115 ;PMID: 29339494

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  • Title:
    Strongly enhanced bacterial bioluminescence with the ilux operon for single-cell imaging
  • Author: Gregor, Carola ; Gwosch, Klaus C. ; Sahl, Steffen J. ; Hell, Stefan W.
  • Subjects: Bacteria ; Biological Sciences ; Bioluminescence ; Bleaching ; Cells ; Coding ; Genetic code ; Molecules ; Phototoxicity ; Physical Sciences ; Substrates
  • Is Part Of: Proceedings of the National Academy of Sciences - PNAS, 2018-01, Vol.115 (5), p.962-967
  • Description: Bioluminescence imaging of single cells is often complicated by the requirement of exogenous luciferins that can be poorly cell-permeable or produce high background signal. Bacterial bioluminescence is unique in that it uses reduced flavin mononucleotide as a luciferin, which is abundant in all cells, making this system purely genetically encodable by the lux operon. Unfortunately, the use of bacterial bioluminescence has been limited by its low brightness compared with other luciferases. Here, we report the generation of an improved lux operon named ilux with an approximately sevenfold increased brightness when expressed in Escherichia coli; ilux can be used to image single E. coli cells with enhanced spatiotemporal resolution over several days. In addition, since only metabolically active cells produce bioluminescent signal, we show that ilux can be used to observe the effect of different antibiotics on cell viability on the single-cell level.
  • Publisher: United States: National Academy of Sciences
  • Language: English
  • Identifier: ISSN: 0027-8424
    EISSN: 1091-6490
    DOI: 10.1073/pnas.1715946115
    PMID: 29339494
  • Source: GFMER Free Medical Journals
    PubMed Central
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