skip to main content
Language:
Search Limited to: Search Limited to: Resource type Show Results with: Show Results with: Search type Index

REGENERACIÓN DE PLANTAS DE Agave marmorata Roelz EN SISTEMAS DE INMERSIÓN TEMPORAL, MEDIANTE ORGANOGÉNESIS Y EMBRIOGÉNESIS SOMÁTICA

Tropical and subtropical agroecosystems, 2021-09, Vol.24 (3) [Peer Reviewed Journal]

ISSN: 1870-0462 ;EISSN: 1870-0462 ;DOI: 10.56369/tsaes.3472

Full text available

Citations Cited by
  • Title:
    REGENERACIÓN DE PLANTAS DE Agave marmorata Roelz EN SISTEMAS DE INMERSIÓN TEMPORAL, MEDIANTE ORGANOGÉNESIS Y EMBRIOGÉNESIS SOMÁTICA
  • Author: Martinez-Martinez, Sandra Yarenssy ; Arzate-Fernandez, Amaury Martin ; Álvarez-Aragon, Carlos ; Martínez-Velasco, Irene ; Norman-Mondragon, Thomas
  • Is Part Of: Tropical and subtropical agroecosystems, 2021-09, Vol.24 (3)
  • Description: Background. The genus Agave has social, cultural, environmental and economic importance in Mexico. Approximately 15 species of agave are used as raw material for the production of mezcal, among them is the Agave marmorata Roelz, which is considered the longest-lived agave for the preparation of this drink because its maturation period ranges from 20 to 35 years. Agave plant tissue culture is a technique that allows obtaining plants by organogenesis (O) and / or somatic embryogenesis (ES) in a short period of time. The quality and quantity of plants propagated by these methods can be favored with the use of Temporary Immersion Systems (SIT). Objective. Establish a protocol for the regeneration of A. marmorata seedlings in SIT, with the methods of direct organogenesis and indirect somatic embryogenesis. Methodology. Two regeneration methods, direct organogenesis and indirect somatic embryogenesis, were evaluated. For the first, the effect of the frequency and number of explants grown in SIT in the sprouting and growth stages of the explant was determined. For the second, the effect of the number of explants grown in SIT on the percentage of germination and the growth rate of somatic embryos was evaluated. Results. The best treatment in the sprouting stage was obtained with 80 explants grown in SIT with an immersion frequency of 8 h and an immersion time of 2 min, with 84% sprouting and 17.5 sprouts / explant of 1 cm in height. The best growth of the explants originated from direct organogenesis was obtained with 180 cultured explants per flask with an immersion frequency of 8 hours and an immersion time of 2 min. On the other hand, in the somatic embryogenesis method, the highest percentage of embryo germination (62.2%) was achieved with 80 somatic embryos cultured in SIT with an immersion frequency of 8 hours. Implications. The results of this research contribute to a better understanding of the regeneration process of direct organogenesis and somatic embryogenesis in temporary immersion systems. This can help to scale the system commercially and to conserve germplasm. Conclusions. It was possible to establish an effective protocol for the regeneration of agave seedlings.
  • Language: English
  • Identifier: ISSN: 1870-0462
    EISSN: 1870-0462
    DOI: 10.56369/tsaes.3472
  • Source: Geneva Foundation Free Medical Journals at publisher websites
    ROAD
    DOAJ Directory of Open Access Journals
Back to results list
Go to Previous pagePrevious Result  7 NextGo to Next page
INSPIRE LIBRARY - TON DUC THANG UNIVERSITY (84-028) 37 755 057 Feedback
19 Nguyen Huu Tho St. Dist.7, HCM thuvien@tdtu.edu.vn

Copyright © 2017 INSPIRE LIBRARY - TON DUC THANG UNIVERSITY

Searching Remote Databases, Please Wait