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Detection of Diatoms by a Combination of Membrane Filtration and Transparentness super(,)

Journal of forensic sciences, 2016-11, Vol.61 (6), p.1643-1646 [Peer Reviewed Journal]

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Title:
Detection of Diatoms by a Combination of Membrane Filtration and Transparentness super(,)
Author: Zhao, Jian ; Wang, Yuzhong ; Liu, Chao ; Hu, Sunlin ; Shi, He ; Wen, Jinfeng
Subjects: Bacillariophyceae
Is Part Of: Journal of forensic sciences, 2016-11, Vol.61 (6), p.1643-1646
Description: Diagnosis of drowning remains a difficult issue in current forensic sciences. A large number of diatoms were lost by removing the supernatant after centrifugation in the conventional forensic diatom test. We developed a novel membrane filtration method to enrich diatoms from samples. A new solution using different ratios of acetic acid and eugenol is prepared to make the membrane transparent. These processes allow the diatom-containing membrane to be visualized and identified easily by light microscopy. The tissues contaminated by water rich in diatoms were detected by the new method for the recovery of diatoms. Eleven drowning cases were analyzed by both the new method and the conventional method to compare the sensitivity of both methods. The recovery of the novel diatom test method was 54.2 plus or minus 23.1%. The positive rate of the novel method has been proven to be superior to the conventional method in the diagnosis of drowning.
Language: English
Identifier: ISSN: 0022-1198
EISSN: 1556-4029
DOI: 10.1111/1556-4029.13181
Source: Alma/SFX Local Collection

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