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SHV-12, SHV-5, SHV-2a and VEB-1 extended-spectrum β-lactamases in Gram-negative bacteria isolated in a university hospital in Thailand

Journal of antimicrobial chemotherapy, 2001-12, Vol.48 (6), p.839-852 [Peer Reviewed Journal]

2002 INIST-CNRS ;ISSN: 0305-7453 ;ISSN: 1460-2091 ;EISSN: 1460-2091 ;DOI: 10.1093/jac/48.6.839 ;PMID: 11733468 ;CODEN: JACHDX

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Title:
SHV-12, SHV-5, SHV-2a and VEB-1 extended-spectrum β-lactamases in Gram-negative bacteria isolated in a university hospital in Thailand
Author: Chanawong, Aroonwadee ; M'Zali, Fatima H. ; Heritage, John ; Lulitanond, Aroonlug ; Hawkey, Peter M.
Subjects: Bacteriology ; beta-Lactamases - genetics ; beta-Lactamases - isolation & purification ; Biological and medical sciences ; Cross Infection - enzymology ; Cross Infection - epidemiology ; Cross Infection - genetics ; Cross Infection - microbiology ; Escherichia coli Proteins ; Fundamental and applied biological sciences. Psychology ; Genetics ; Gram-Negative Bacteria - genetics ; Gram-Negative Bacteria - isolation & purification ; Gram-Negative Bacterial Infections - enzymology ; Gram-Negative Bacterial Infections - epidemiology ; Gram-Negative Bacterial Infections - genetics ; Hospitals, University - statistics & numerical data ; Humans ; Microbiology ; Molecular Sequence Data ; Thailand - epidemiology
Is Part Of: Journal of antimicrobial chemotherapy, 2001-12, Vol.48 (6), p.839-852
Description: Sixty-one extended-spectrum β-lactamase (ESBL)-producing isolates were collected from Srinagarind Hospital, Thailand. These included 43 Enterobacteriaceae and 18 Pseudomonadaceae. The 43 Enterobacteriaceae were found to produce the following ESBLs: 26 (60.5%) SHV-12, 13 (30.2%) SHV-5, two (4.7%) SHV-2a, one (2.3%) VEB-1 and one (2.3%) unidentified. Twenty-four isolates (55.8%) also carried blaTEM-1B, as well as blaSHV or blaVEB-1. Plasmid DNA from transconjugants carrying the blaSHV-12 gene showed various restriction patterns, indicating the distribution of the blaSHV-12 gene among different antibiotic resistance plasmids. In contrast, blaSHV-5 in 13 isolates was found on a single plasmid of c. 130 kb. Pulsed-field gel electrophoresis (PFGE) analysis of genomic DNA from these isolates revealed that nine of 11 Klebsiella pneumoniae gave the same pattern, indicating clonal spread of the strain within the hospital, together with the occasional spread of the plasmid to other strains. Among the pseudomonad isolates, 16 Pseudomonas aeruginosa and one Pseudomonas putida had blaVEB-like and one P. aeruginosa had blaSHV-12. Nine of the 16 isolates carrying blaVEB-like (56.3%) had identical PFGE patterns, suggesting the dissemination of this gene, also by clonal spread. At least six different blaVEB-like-containing integrons were found among the 18 isolates. This is the first report of bacteria producing SHV-12 and SHV-2a in Thailand and the first report of SHV-12 in P. aeruginosa, of VEB-1 in Citrobacter freundii and a VEB-1-like β-lactamase in P. putida. These findings indicate that ESBL genes in the Far East are part of a gene pool capable of broad horizontal gene transfer, in that these genes can transfer between different families of Gram-negative bacilli.
Publisher: Oxford: Oxford University Press
Language: English
Identifier: ISSN: 0305-7453
ISSN: 1460-2091
EISSN: 1460-2091
DOI: 10.1093/jac/48.6.839
PMID: 11733468
CODEN: JACHDX
Source: GFMER Free Medical Journals
MEDLINE
Alma/SFX Local Collection

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SHV-12, SHV-5, SHV-2a and VEB-1 extended-spectrum β-lactamases in Gram-negative bacteria isolated in a university hospital in Thailand

2002 INIST-CNRS ;ISSN: 0305-7453 ;ISSN: 1460-2091 ;EISSN: 1460-2091 ;DOI: 10.1093/jac/48.6.839 ;PMID: 11733468 ;CODEN: JACHDX

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