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Hydrolysis of extracted and fibre-bound xylan with Aureobasidium pullulans enzymes

Journal of biotechnology, 1997-01, Vol.55 (1), p.21-29 [Peer Reviewed Journal]

1997 Elsevier Science B.V. ;1997 INIST-CNRS ;ISSN: 0168-1656 ;EISSN: 1873-4863 ;DOI: 10.1016/S0168-1656(97)00048-5 ;CODEN: JBITD4

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  • Title:
    Hydrolysis of extracted and fibre-bound xylan with Aureobasidium pullulans enzymes
  • Author: Christov, L.P ; Myburgh, J ; van Tonder, A ; Prior, B.A
  • Subjects: Aureobasidium pullulans ; Biological and medical sciences ; Dissolving pulp ; Endo-xylanase ; Enzyme hydrolysis ; Fundamental and applied biological sciences. Psychology ; Growth, nutrition, metabolism, transports, enzymes. Molecular biology ; Microbiology ; Mycology ; O-glycoside hydrolases ; wood pulp ; xylan 1,4-beta-xylosidase ; Xylan accessibility ; xylanase ; β-Xylosidase
  • Is Part Of: Journal of biotechnology, 1997-01, Vol.55 (1), p.21-29
  • Description: The enzymatic hydrolysis of arabinoxylan extracted from oat spelts and fibre-bound xylan present in dissolving pulp was investigated and compared using purified β-xylanase and β-xylosidase as well as the crude enzyme preparation of the yeast Aureobasidium pullulans. By means of size-exclusion and anion exchange chromatography both enzymes were purified to homogeneity with apparent molecular masses of 20 kDa ( β-xylanase) and 216 kDa ( β-xylosidase). Addition of greater activities of β-xylosidase (up to 1.5 IU g −1 of araboinoxylan) to β-xylanase (1.5 IU g −1 of araboinoxylan) in the enzyme-arabinoxylan reaction mixture resulted in the amount and rate of release of xylose and xylo-oligomers being increased. In the absence of β-xylosidase only xylobiose, xylotriose and higher oligomers were detected by high performance liquid chromatography. When β-xylanase or β-xylosidase or a β-xylanase/ β-xylosidase mixture was added to dissolving pulp, release of xylose and xylo-oligomers (xylobiose, xylotriose and higher oligomers) was observed in all instances using thin-layer chromatography. The results showed that the purified and crude enzymes of A. pullulans were only partially effective in the removal of xylan from dissolving pulp and the hydrolysis reaction was both time- and substrate-dependent.
  • Publisher: Lausanne: Elsevier B.V
  • Language: English
  • Identifier: ISSN: 0168-1656
    EISSN: 1873-4863
    DOI: 10.1016/S0168-1656(97)00048-5
    CODEN: JBITD4
  • Source: Alma/SFX Local Collection
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