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Enzyme activity recovery from secondary fiber treated with cellulase and xylanase
Journal of biotechnology, 1996-01, Vol.45 (1), p.33-44
[Peer Reviewed Journal]
1996 ;1996 INIST-CNRS ;ISSN: 0168-1656 ;EISSN: 1873-4863 ;DOI: 10.1016/0168-1656(95)00137-9 ;CODEN: JBITD4
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Title:
Enzyme activity recovery from secondary fiber treated with cellulase and xylanase
Author:
Jackson, L.S.
;
Joyce, T.W.
;
Heitmann, J.A.
;
Giesbrecht, F.G.
Subjects:
Biological and medical sciences
;
Biotechnology
;
bleaching
;
Cellulase
;
cellulases
;
enzyme activity
;
Enzyme engineering
;
Enzyme recovery
;
Fundamental and applied biological sciences. Psychology
;
hardwood
;
kraft pulping
;
Methods. Procedures. Technologies
;
Miscellaneous
;
nonionic surfactants
;
O-glycoside hydrolases
;
recovery
;
Secondary fiber
;
softwood
;
tween 80
;
wood pulp
;
Xylanase
Is Part Of:
Journal of biotechnology, 1996-01, Vol.45 (1), p.33-44
Description:
One of the major problems with implementing biotechnical processes in the recycled paper industry, such as enzyme-enhanced deinking and enzymatic enhancement of pulp drainage properties, is the cost of commercial enzyme preparations. Thus, several factorial studies were performed to determine if enzyme activity can be successfully removed from simulated recycled fiber (once-dried, bleached hardwood and softwood kraft fiber) treated with low concentrations (0.2% or 2.0% on oven-dry fiber) of cellulase or xylanase. Enzyme activity recovery was accomplished by washing treated fiber with dilute NaOH in combination with a low concentration of the nonionic surfactant Tween 80 under a variety of mild conditions. Various cellulase activities i.e., endoglucanase, exoglucanase, and filter paper, can be effectively recovered depending on the washing conditions, the cellulase charge, and the fiber type. Xylanase activity was effectively recovered from softwood, but not hardwood. The results suggest that enzyme activity recovery may be a possible means of decreasing the operating costs for biotechnical processes in the paper industry.
Publisher:
Lausanne: Elsevier B.V
Language:
English
Identifier:
ISSN: 0168-1656
EISSN: 1873-4863
DOI: 10.1016/0168-1656(95)00137-9
CODEN: JBITD4
Source:
Alma/SFX Local Collection
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