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1,1-dichloroethylene-induced alterations in glutathione and covalent binding in murine lung: Morphological, histochemical, and biochemical studies

The Journal of pathology, 1992-02, Vol.166 (2), p.199-207 [Peer Reviewed Journal]

Copyright © 1992 John Wiley & Sons, Ltd. ;1992 INIST-CNRS ;ISSN: 0022-3417 ;EISSN: 1096-9896 ;DOI: 10.1002/path.1711660218 ;PMID: 1560321 ;CODEN: JPTLAS

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Title:
1,1-dichloroethylene-induced alterations in glutathione and covalent binding in murine lung: Morphological, histochemical, and biochemical studies
Author: Moussa, Madeleine ; Forkert, Poh-Gek
Subjects: 1 ; Animals ; Biological and medical sciences ; Chemical and industrial products toxicology. Toxic occupational diseases ; Chemical Phenomena ; Chemistry ; Clara cell ; covalent binding ; Dichloroethylene ; Dichloroethylenes - adverse effects ; Dichloroethylenes - pharmacology ; Dose-Response Relationship, Drug ; Epithelium - ultrastructure ; Gas, fumes ; glutathione ; Glutathione - analysis ; Glutathione - metabolism ; Immunohistochemistry ; Lung - chemistry ; Lung - drug effects ; Lung - metabolism ; Lung - ultrastructure ; Male ; Medical sciences ; Mice ; Mice, Inbred Strains ; Microscopy, Electron ; Microscopy, Electron, Scanning ; Toxicology
Is Part Of: The Journal of pathology, 1992-02, Vol.166 (2), p.199-207
Description: Non‐ciliated Clara cells of the pulmonary bronchiolar epithelium are preferentially damaged by administration of 1,1‐dichloroethylene (1,1‐DCE) to mice. In this study, an in vivo system was utilized to investigate the dose‐dependent effects of 1,1‐DCE (75, 125, 175, and 225 mg/kg) on covalent binding and on reduced glutathione (GSH) in murine lung. Treatment of mice with each dose level of 1,1‐DCE elicited significant decreases in GSH content and resulted in covalent binding of [14C]1,1‐DCE in a dose‐dependent manner. Histochemical staining for GSH in lungs of control mice revealed positive cellular sites in alveolar septa and bronchiolar epithelium, with the highest staining intensities in Clara cells. Staining was reduced after exposure to 75 and 125 mg/kg 1,1‐DCE, and at higher doses it was abolished in alveolar septa and retained in bronchiolar epithelium, albeit at considerably reduced intensities. Heterogeneity with respect to staining intensities was consistently observed in the Clara cell population in both control and 1,1‐DCE‐treated mice. Progressive increases in covalent binding and decreases in GSH content correlated with increasing severities of Clara cell injury. These results show a dose dependence in regard to the magnitudes of [14C]1,1‐DCE binding, the alterations in cellular GSH, and the severities of Clara cell necrosis.
Publisher: Chichester, UK: John Wiley & Sons, Ltd
Language: English
Identifier: ISSN: 0022-3417
EISSN: 1096-9896
DOI: 10.1002/path.1711660218
PMID: 1560321
CODEN: JPTLAS
Source: MEDLINE
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1,1-dichloroethylene-induced alterations in glutathione and covalent binding in murine lung: Morphological, histochemical, and biochemical studies

Copyright © 1992 John Wiley & Sons, Ltd. ;1992 INIST-CNRS ;ISSN: 0022-3417 ;EISSN: 1096-9896 ;DOI: 10.1002/path.1711660218 ;PMID: 1560321 ;CODEN: JPTLAS

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