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TCP / Method for manufacturing bone-induction bilayer membrane containing electrospun polycaprolactone/gelatin/-TCP mat and alginate/gelatin hydrogel
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Title:
TCP / Method for manufacturing bone-induction bilayer membrane containing electrospun polycaprolactone/gelatin/-TCP mat and alginate/gelatin hydrogel
Author:
LEE BYONG TAEK
;
PADALHIN ANDREW
;
HO VAN HAI
;
LE THI THANH THAO
Subjects:
BANDAGES, DRESSINGS OR ABSORBENT PADS
;
CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, ORSURGICAL ARTICLES
;
DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF,TUBULAR STRUCTURES OF THE BODY, E.G. STENTS
;
DISINFECTION, STERILISATION, OR DEODORISATION OF AIR
;
FILTERS IMPLANTABLE INTO BLOOD VESSELS
;
FIRST-AID KITS
;
FOMENTATION
;
HUMAN NECESSITIES
;
HYGIENE
;
MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICALARTICLES
;
MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OFARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES ORRIBBONS
;
MEDICAL OR VETERINARY SCIENCE
;
METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS INGENERAL
;
NATURAL OR ARTIFICIAL THREADS OR FIBRES
;
ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES
;
PROSTHESES
;
SPINNING
;
TEXTILES
;
TREATMENT OR PROTECTION OF EYES OR EARS
Description:
A bilayer membrane including sodium alginate/gelatin, of which electrospun polycaprolactone/gelatin/β-phosphate tricalcium phosphate nanopowder is coated on one surface, is synthetized and evaluated for application in bone regeneration. A main objective of the present invention is not only to induce bone regeneration but also to manufacture a composite membrane that has strong tensile strength and prevents migration of soft tissues by bone defects. A role of SA/gel coating surface in the membrane is to improve wound covering and prevent migration of soft tissues while the porous electrospun layer supports bone cell growth and bone regeneration activity. Cell viability and cell proliferation were obtained by MTT analysis and SEM images of MCT3T3 and L929 cells. Effects with or without bilayer membrane treatment on rabbit radius bones are compared at a period of two weeks and four weeks. As a result of observation by micro-CT reconstructed images by N-recon software, the bilayer structure membrane showed good mechanical strength and bone induction after four weeks. 전기 방사된 폴리카프로락톤/젤라틴/β-인삼3칼슘포스페이트 나노 분말이 1표면에 코팅된 알긴산 나트륨/젤라틴을 포함하는 이중층 막을 합성하고 골 재생 적용에 대해 평가하였다. 본 발명의 주요 목적은 뼈 재생을 유도할 뿐만 아니라 인장 강도가 충분히 강하고 뼈 결함으로 연조직 이동을 방지하는 유용한 복합 막을 제조하는 것입니다. 멤브레인에서 SA/겔 코팅 표면의 역할은 다공성 전기 방사 층이 뼈 세포 성장을 지원하고 뼈 재생 활동을 촉진하는 반면 상처 덮음을 향상시키고 연조직 이동을 방지하는 것입니다. MCT3T3 및 L929 세포의 MTT 분석 및 SEM 이미지에 의해 얻어진 세포 생존력 및 세포 증식력을 얻었다. 토끼 반경 뼈에 대한 이중층 막 처리가 있거나 없는 경우에 효과를 2 주 및 4 주에 비교 하였다. N-recon 소프트웨어에 의한 미세 CT 재구성 영상에 의해 관찰한 결과, 이중층 구조 막은 4 주 후에 양호한 기계적 강도 및 뼈 유도를 나타내었다.
Creation Date:
2020
Language:
English;Korean
Source:
esp@cenet
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