skip to main content
Language:
Search Limited to: Search Limited to: Resource type Show Results with: Show Results with: Search type Index

Metagenomic and network analysis reveal wide distribution and co-occurrence of environmental antibiotic resistance genes

The ISME Journal, 2015-11, Vol.9 (11), p.2490-2502 [Peer Reviewed Journal]

Copyright Nature Publishing Group Nov 2015 ;Copyright © 2015 International Society for Microbial Ecology 2015 International Society for Microbial Ecology ;ISSN: 1751-7362 ;EISSN: 1751-7370 ;DOI: 10.1038/ismej.2015.59 ;PMID: 25918831

Full text available

Citations Cited by
  • Title:
    Metagenomic and network analysis reveal wide distribution and co-occurrence of environmental antibiotic resistance genes
  • Author: Li, Bing ; Yang, Ying ; Ma, Liping ; Ju, Feng ; Guo, Feng ; Tiedje, James M ; Zhang, Tong
  • Subjects: Animals ; Anti-Bacterial Agents - chemistry ; Chickens ; Drug Resistance, Microbial - genetics ; Feces ; Gene Dosage ; Genes, Bacterial ; Humans ; Metagenomics ; Original ; RNA, Ribosomal, 16S - genetics ; Swine ; Tetracycline Resistance - genetics ; Vancomycin Resistance - genetics ; Waste Water
  • Is Part Of: The ISME Journal, 2015-11, Vol.9 (11), p.2490-2502
  • Description: A metagenomic approach and network analysis was used to investigate the wide-spectrum profiles of antibiotic resistance genes (ARGs) and their co-occurrence patterns in 50 samples from 10 typical environments. In total, 260 ARG subtypes belonging to 18 ARG types were detected with an abundance range of 5.4 × 10(-6)-2.2 × 10(-1) copy of ARG per copy of 16S-rRNA gene. The trend of the total ARG abundances in environments matched well with the levels of anthropogenic impacts on these environments. From the less impacted environments to the seriously impacted environments, the total ARG abundances increased up to three orders of magnitude, that is, from 3.2 × 10(-3) to 3.1 × 10(0) copy of ARG per copy of 16S-rRNA gene. The abundant ARGs were associated with aminoglycoside, bacitracin, β-lactam, chloramphenicol, macrolide-lincosamide-streptogramin, quinolone, sulphonamide and tetracycline, in agreement with the antibiotics extensively used in human medicine or veterinary medicine/promoters. The widespread occurrences and abundance variation trend of vancomycin resistance genes in different environments might imply the spread of vancomycin resistance genes because of the selective pressure resulting from vancomycin use. The simultaneous enrichment of 12 ARG types in adult chicken faeces suggests the coselection of multiple ARGs in this production system. Non-metric multidimensional scaling analysis revealed that samples belonging to the same environment generally possessed similar ARG compositions. Based on the co-occurrence pattern revealed by network analysis, tetM and aminoglycoside resistance protein, the hubs of the ARG network, are proposed to be indicators to quantitatively estimate the abundance of 23 other co-occurring ARG subtypes by power functions.
  • Publisher: England: Nature Publishing Group
  • Language: English
  • Identifier: ISSN: 1751-7362
    EISSN: 1751-7370
    DOI: 10.1038/ismej.2015.59
    PMID: 25918831
  • Source: MEDLINE
    PubMed Central
    ProQuest Central
Back to results list
INSPIRE LIBRARY - TON DUC THANG UNIVERSITY (84-028) 37 755 057 Feedback
19 Nguyen Huu Tho St. Dist.7, HCM thuvien@tdtu.edu.vn

Copyright © 2017 INSPIRE LIBRARY - TON DUC THANG UNIVERSITY

Searching Remote Databases, Please Wait