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Development of Simple Analytical Method for B-Group Vitamins in Nutritional Products: Enzymatic Digestion and UPLC-MS/MS Quantification

Journal of analytical methods in chemistry, 2021-05, Vol.2021, p.1-10 [Peer Reviewed Journal]

Copyright © 2021 Quang Huy Nguyen et al. ;COPYRIGHT 2021 Hindawi Limited ;Copyright © 2021 Quang Huy Nguyen et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0 ;Copyright © 2021 Quang Huy Nguyen et al. 2021 ;ISSN: 2090-8865 ;EISSN: 2090-8873 ;DOI: 10.1155/2021/5526882 ;PMID: 34035973

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Title:
Development of Simple Analytical Method for B-Group Vitamins in Nutritional Products: Enzymatic Digestion and UPLC-MS/MS Quantification
Author: Nguyen, Quang Huy ; Hoang, Anh Quoc ; Truong, Thi My Hanh ; Dinh, Thi Diu ; Le, Thi Thuy ; Luu, Thi Huyen Trang ; Dinh, Viet Chien ; Nguyen, Thi Minh Thu ; Vu, Thi Trang ; Nguyen, Thi Anh Huong
Moldoveanu, Serban C. ; Serban C Moldoveanu
Subjects: Acids ; Buffer solutions ; Chromatography ; Digestion ; Efficiency ; Enzymes ; Experiments ; Food ; Gas flow ; Heat treatment ; Mass spectrometry ; Methods ; Multiple criterion ; Niacinamide ; Nicotinamide ; Nutrition ; Optimization ; Phosphatase ; Pyridoxine ; Reagents ; Reproducibility ; Riboflavin ; Scientific imaging ; Selectivity ; Thiamine ; Vitamin deficiency ; Vitamins
Is Part Of: Journal of analytical methods in chemistry, 2021-05, Vol.2021, p.1-10
Description: A method for the simultaneous determination of seven B-group vitamers including thiamine, riboflavin, nicotinamide, niacin, pyridoxine, pyridoxal, and pyridoxamine in nutritional products by using enzymatic digestion followed by LC-MS/MS quantification was studied. The LC-MS/MS conditions such as MS transitions, mobile phase programs, and ammonium formate buffer concentrations, and sample treatment procedures (e.g., concentrations of buffer solution, digestion temperature, and digestion time) were investigated. The analytical method performance was evaluated by multiple criteria such as selectivity, linearity, detection and quantification limits, repeatability, reproducibility, and recovery by using real sample matrices. The validated method was successfully applied to analyze vitamin B concentrations in different nutritional products like ultra-heat-treated milk, powdered milk, and nutritional powder. Vitamin B concentrations varied over a wide range from lower than detection limits to about 9000 µg/100 g, depending on vitamin groups, compound forms, and sample types. The measured concentrations of B-group vitamins in our samples were generally in good agreement with values of label claims.
Publisher: New York: Hindawi
Language: English
Identifier: ISSN: 2090-8865
EISSN: 2090-8873
DOI: 10.1155/2021/5526882
PMID: 34035973
Source: GFMER Free Medical Journals
PubMed Central
ProQuest Central
DOAJ Directory of Open Access Journals

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Development of Simple Analytical Method for B-Group Vitamins in Nutritional Products: Enzymatic Digestion and UPLC-MS/MS Quantification

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