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Synthetic xylan-binding modules for mapping of pulp fibres and wood sections

BMC plant biology, 2007-10, Vol.7 (1), p.54-54, Article 54 [Peer Reviewed Journal]

COPYRIGHT 2007 BioMed Central Ltd. ;Copyright © 2007 Filonova et al; licensee BioMed Central Ltd. 2007 Filonova et al; licensee BioMed Central Ltd. ;ISSN: 1471-2229 ;EISSN: 1471-2229 ;DOI: 10.1186/1471-2229-7-54 ;PMID: 17935619

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Title:
Synthetic xylan-binding modules for mapping of pulp fibres and wood sections
Author: Filonova, Lada ; Gunnarsson, Lavinia Cicortas ; Daniel, Geoffrey ; Ohlin, Mats
Subjects: Bacterial Proteins - chemistry ; Bacterial Proteins - metabolism ; Basic Medicine ; Betula - chemistry ; Betula - metabolism ; Carbohydrate Metabolism ; Carbohydrates ; Cell differentiation ; Cellulose ; Chemical properties ; Evaluation ; Immunologi inom det medicinska området ; Immunology in the medical area ; Medical and Health Sciences ; Medicin och hälsovetenskap ; Medicinska och farmaceutiska grundvetenskaper ; Microscopy, Fluorescence ; Physiological aspects ; Populus - chemistry ; Populus - metabolism ; Pulp industry ; Rhodothermus - enzymology ; Sequence Analysis, Protein ; Wood - chemistry ; Wood - metabolism ; Xylanases ; Xylans - analysis ; Xylans - chemistry ; Xylans - metabolism
Is Part Of: BMC plant biology, 2007-10, Vol.7 (1), p.54-54, Article 54
Description: The complex carbohydrate composition of natural and refined plant material is not known in detail but a matter that is of both basic and applied importance. Qualitative assessment of complex samples like plant and wood tissues requires the availability of a range of specific probes. Monoclonal antibodies and naturally existing carbohydrate binding modules (CBMs) have been used in the past to assess the presence of certain carbohydrates in plant tissues. However, the number of natural CBMs is limited and development of carbohydrate-specific antibodies is not always straightforward. We envisage the use of sets of very similar proteins specific for defined targets, like those developed by molecular evolution of a single CBM scaffold, as a suitable strategy to assess carbohydrate composition. An advantage of using synthetic CBMs lies in the possibility to study fine details of carbohydrate composition within non-uniform substrates like plant cell walls as made possible through minor differences in CBM specificity of the variety of binders that can be developed by genetic engineering. A panel of synthetic xylan-binding CBMs, previously selected from a molecular library based on the scaffold of CBM4-2 from xylanase Xyn10A of Rhodothermus marinus, was used in this study. The wild type CBM4-2 and evolved modules both showed binding to wood sections. However, differences were observed in the staining patterns suggesting that these modules have different xylan-binding properties. Also the staining stability varied between the CBMs, the most stable staining being obtained with one (X-2) of the synthetic modules. Treatment of wood materials resulted in altered signal intensities, thereby also demonstrating the potential application of engineered CBMs as analytical tools for quality assessment of diverse plant material processes. In this study we have demonstrated the usefulness of synthetic xylan-binding modules as specific probes in analysis of hemicelluloses (xylan) in wood and fibre materials.
Publisher: England: BioMed Central Ltd
Language: English
Identifier: ISSN: 1471-2229
EISSN: 1471-2229
DOI: 10.1186/1471-2229-7-54
PMID: 17935619
Source: Freely Accessible Journals
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Synthetic xylan-binding modules for mapping of pulp fibres and wood sections

COPYRIGHT 2007 BioMed Central Ltd. ;Copyright © 2007 Filonova et al; licensee BioMed Central Ltd. 2007 Filonova et al; licensee BioMed Central Ltd. ;ISSN: 1471-2229 ;EISSN: 1471-2229 ;DOI: 10.1186/1471-2229-7-54 ;PMID: 17935619

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