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Extended insight into the Mycobacterium chelonae-abscessus complex through whole genome sequencing of Mycobacterium salmoniphilum outbreak and Mycobacterium salmoniphilum-like strains

Scientific reports, 2019-03, Vol.9 (1), p.4603-4603, Article 4603 [Peer Reviewed Journal]

This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. ;The Author(s) 2019 ;ISSN: 2045-2322 ;EISSN: 2045-2322 ;DOI: 10.1038/s41598-019-40922-x ;PMID: 30872669

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Title:
Extended insight into the Mycobacterium chelonae-abscessus complex through whole genome sequencing of Mycobacterium salmoniphilum outbreak and Mycobacterium salmoniphilum-like strains
Author: Behra, Phani Rama Krishna ; Das, Sarbashis ; Pettersson, B M Fredrik ; Shirreff, Lisa ; DuCote, Tanner ; Jacobsson, Karl-Gustav ; Ennis, Don G ; Kirsebom, Leif A
Subjects: Animals ; Computational Biology - methods ; Cytotoxicity ; Genome, Bacterial ; Genomes ; Genomics - methods ; Humans ; Macrophages ; Molecular Sequence Annotation ; Mycobacterium ; Mycobacterium - genetics ; Mycobacterium abscessus - genetics ; Mycobacterium chelonae ; Mycobacterium Infections - microbiology ; Mycobacterium Infections, Nontuberculous - microbiology ; Outbreaks ; Pathogens ; Phospholipase C ; Phylogeny ; Polymorphism, Single Nucleotide ; RNA, Ribosomal, 16S - genetics ; Sigma factor ; Transcription ; tRNA ; Virulence factors ; Whole Genome Sequencing
Is Part Of: Scientific reports, 2019-03, Vol.9 (1), p.4603-4603, Article 4603
Description: Members of the Mycobacterium chelonae-abscessus complex (MCAC) are close to the mycobacterial ancestor and includes both human, animal and fish pathogens. We present the genomes of 14 members of this complex: the complete genomes of Mycobacterium salmoniphilum and Mycobacterium chelonae type strains, seven M. salmoniphilum isolates, and five M. salmoniphilum-like strains including strains isolated during an outbreak in an animal facility at Uppsala University. Average nucleotide identity (ANI) analysis and core gene phylogeny revealed that the M. salmoniphilum-like strains are variants of the human pathogen Mycobacterium franklinii and phylogenetically close to Mycobacterium abscessus. Our data further suggested that M. salmoniphilum separates into three branches named group I, II and III with the M. salmoniphilum type strain belonging to group II. Among predicted virulence factors, the presence of phospholipase C (plcC), which is a major virulence factor that makes M. abscessus highly cytotoxic to mouse macrophages, and that M. franklinii originally was isolated from infected humans make it plausible that the outbreak in the animal facility was caused by a M. salmoniphilum-like strain. Interestingly, M. salmoniphilum-like was isolated from tap water suggesting that it can be present in the environment. Moreover, we predicted the presence of mutational hotspots in the M. salmoniphilum isolates and 26% of these hotspots overlap with genes categorized as having roles in virulence, disease and defense. We also provide data about key genes involved in transcription and translation such as sigma factor, ribosomal protein and tRNA genes.
Publisher: England: Nature Publishing Group
Language: English
Identifier: ISSN: 2045-2322
EISSN: 2045-2322
DOI: 10.1038/s41598-019-40922-x
PMID: 30872669
Source: MEDLINE
PubMed Central
SWEPUB Freely available online
ProQuest Central
DOAJ Directory of Open Access Journals

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Extended insight into the Mycobacterium chelonae-abscessus complex through whole genome sequencing of Mycobacterium salmoniphilum outbreak and Mycobacterium salmoniphilum-like strains

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