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Serum CTX levels and histomorphometric analysis in Src versus RANKL knockout mice

Journal of bone and mineral metabolism, 2018-05, Vol.36 (3), p.264-273 [Peer Reviewed Journal]

The Japanese Society for Bone and Mineral Research and Springer Japan 2017 ;Journal of Bone and Mineral Metabolism is a copyright of Springer, (2017). All Rights Reserved. ;ISSN: 0914-8779 ;EISSN: 1435-5604 ;DOI: 10.1007/s00774-017-0838-3 ;PMID: 28589412

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Title:
Serum CTX levels and histomorphometric analysis in Src versus RANKL knockout mice
Author: Takeshita, Sunao ; Fumoto, Toshio ; Ito, Masako ; Ikeda, Kyoji
Subjects: Animals ; Biomarkers - metabolism ; Bone histomorphometry ; Bone Resorption - blood ; Bone Resorption - genetics ; Bone Resorption - metabolism ; Collagen ; Collagen Type I - blood ; Computed tomography ; Dentin ; Gene dosage ; Gene Expression Regulation ; Imaging, Three-Dimensional ; Medicine ; Medicine & Public Health ; Metabolic Diseases ; Mice ; Mice, Knockout ; Original Article ; Orthopedics ; Osteoblasts ; Osteoblasts - metabolism ; Osteocalcin - blood ; Osteoclasts ; Osteoclasts - metabolism ; Osteocytes ; Osteocytes - metabolism ; Osteoid ; Osteopetrosis ; Peptides - blood ; RANK Ligand - deficiency ; Rodents ; src-Family Kinases - deficiency ; Tartrate-Resistant Acid Phosphatase - blood ; Tibia - diagnostic imaging ; Tibia - pathology ; TRANCE protein ; X-Ray Microtomography
Is Part Of: Journal of bone and mineral metabolism, 2018-05, Vol.36 (3), p.264-273
Description: Src knockout (KO) and RANKL KO mice both exhibit near complete osteopetrosis in terms of 3D-bone volume (BV) fraction by micro-CT, whereas the serum CTX concentration of Src KO is apparently normal and that of RANKL KO is 30% of wild-type (WT) despite the fact that they lack osteoclasts. By histomorphometry we found that, whereas eroded surface (ES) and osteoid surface (OS) are zero values in RANKL KO, they are indistinguishable from WT in Src KO; because of marked increase in bone surface (BS), ES/BS and OS/BS of Src KO are 30–40% of WT. While RANKL KO lack both osteoclasts and osteoblasts, Src KO reveal increased numbers of osteoclasts and indistinguishable numbers of osteoblasts compared with WT; again, on the basis of BS, N.Oc/BS is comparable to WT and N.Ob/BS is markedly decreased in Src KO. The apparently increased number of total osteoclasts may be due to increased expression of RANKL found in Src KO bone in vivo. Src has a gene dosage-dependent effect on osteoclast function in vitro, with Src −/− osteoclasts completely lacking bone-resorbing function as determined by CTX release on dentin. Thus, Src KO osteoclasts retain some bone-resorbing function in vivo. The number of osteocytes is proportionally increased in RANKL KO, while Src KO mice have relative osteocyte deficiency, raising the possibility that RANKL and Src has an unrecognized role in osteocyte survival.
Publisher: Tokyo: Springer Japan
Language: English
Identifier: ISSN: 0914-8779
EISSN: 1435-5604
DOI: 10.1007/s00774-017-0838-3
PMID: 28589412
Source: MEDLINE
ProQuest Central

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Serum CTX levels and histomorphometric analysis in Src versus RANKL knockout mice

The Japanese Society for Bone and Mineral Research and Springer Japan 2017 ;Journal of Bone and Mineral Metabolism is a copyright of Springer, (2017). All Rights Reserved. ;ISSN: 0914-8779 ;EISSN: 1435-5604 ;DOI: 10.1007/s00774-017-0838-3 ;PMID: 28589412

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