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Nucleosome Repeat Length and Linker Histone Stoichiometry Determine Chromatin Fiber Structure

Proceedings of the National Academy of Sciences - PNAS, 2008-07, Vol.105 (26), p.8872-8877 [Peer Reviewed Journal]

Copyright 2008 The National Academy of Sciences of the United States of America ;Copyright National Academy of Sciences Jul 1, 2008 ;2008 by The National Academy of Sciences of the USA ;ISSN: 0027-8424 ;EISSN: 1091-6490 ;DOI: 10.1073/pnas.0802336105 ;PMID: 18583476

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Title:
Nucleosome Repeat Length and Linker Histone Stoichiometry Determine Chromatin Fiber Structure
Author: Routh, Andrew ; Sandin, Sara ; Rhodes, Daniela
Subjects: Animals ; Base Pairing ; Biochemistry ; Biological Sciences ; Chickens ; Chromatin ; Deoxyribonucleic acid ; DNA ; Gels ; Histones ; Histones - metabolism ; Modeling ; Molecular structure ; Molecules ; Nuclear models ; Nucleosomes ; Nucleosomes - chemistry ; Nucleosomes - metabolism ; Nucleosomes - ultrastructure ; Nucleotide sequences ; Protein Binding ; Proteins ; Repetitive Sequences, Nucleic Acid ; Stoichiometry ; Studies
Is Part Of: Proceedings of the National Academy of Sciences - PNAS, 2008-07, Vol.105 (26), p.8872-8877
Description: To understand how nuclear processes involving DNA are regulated, knowledge of the determinants of chromatin condensation is required. From recent structural studies it has been concluded that the formation of the 30-nm chromatin fiber does not require the linker histone. Here, by comparing the linker histone-dependent compaction of long, reconstituted nucleosome arrays with different nucleosome repeat lengths (NRLs), 167 and 197 bp, we establish that the compaction behavior is both NRL- and linker histone-dependent. Only the 197-bp NRL array can form 30-nm higher-order chromatin structure. Importantly for understanding the regulation of compaction, this array shows a cooperative linker histone-dependent compaction. The 167-bp NRL array displays a limited linker histone-dependent compaction, resulting in a thinner and topologically different fiber. These observations provide an explanation for the distribution of NRLs found in nature.
Publisher: United States: National Academy of Sciences
Language: English
Identifier: ISSN: 0027-8424
EISSN: 1091-6490
DOI: 10.1073/pnas.0802336105
PMID: 18583476
Source: GFMER Free Medical Journals
MEDLINE
PubMed Central

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Nucleosome Repeat Length and Linker Histone Stoichiometry Determine Chromatin Fiber Structure

Copyright 2008 The National Academy of Sciences of the United States of America ;Copyright National Academy of Sciences Jul 1, 2008 ;2008 by The National Academy of Sciences of the USA ;ISSN: 0027-8424 ;EISSN: 1091-6490 ;DOI: 10.1073/pnas.0802336105 ;PMID: 18583476

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