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Assembly mechanism of recombinant spider silk proteins

Proceedings of the National Academy of Sciences - PNAS, 2008-05, Vol.105 (18), p.6590-6595 [Peer Reviewed Journal]

Copyright 2008 The National Academy of Sciences of the United States of America ;Copyright National Academy of Sciences May 6, 2008 ;2008 by The National Academy of Sciences of the USA ;ISSN: 0027-8424 ;EISSN: 1091-6490 ;DOI: 10.1073/pnas.0709246105 ;PMID: 18445655

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Title:
Assembly mechanism of recombinant spider silk proteins
Author: Rammensee, S ; Slotta, U ; Scheibel, T ; Bausch, A.R
Subjects: Aggregation ; Animals ; Biological Sciences ; Biophysics ; Colloids ; Fibers ; Fibroins - chemistry ; Laminar flow ; Liquids ; Microfluidic devices ; Microfluidics ; Models, Biological ; Potassium phosphates ; Protein Engineering ; Protein Structure, Quaternary ; Protein Structure, Secondary ; Proteins ; Recombinant Proteins - chemistry ; Rheology ; Silk ; Silk - chemistry ; Spiders ; Spiders - chemistry
Is Part Of: Proceedings of the National Academy of Sciences - PNAS, 2008-05, Vol.105 (18), p.6590-6595
Description: Spider silk threads are formed by the irreversible aggregation of silk proteins in a spinning duct with dimensions of only a few micrometers. Here, we present a microfluidic device in which engineered and recombinantly produced spider dragline silk proteins eADF3 (engineered Araneus diadematus fibroin) and eADF4 are assembled into fibers. Our approach allows the direct observation and identification of the essential parameters of dragline silk assembly. Changes in ionic conditions and pH result in aggregation of the two proteins. Assembly of eADF3 fibers was induced only in the presence of an elongational flow component. Strikingly, eADF4 formed fibers only in combination with eADF3. On the basis of these results, we propose a model for dragline silk aggregation and early steps of fiber assembly in the microscopic regime.
Publisher: United States: National Academy of Sciences
Language: English
Identifier: ISSN: 0027-8424
EISSN: 1091-6490
DOI: 10.1073/pnas.0709246105
PMID: 18445655
Source: GFMER Free Medical Journals
MEDLINE
PubMed Central

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Assembly mechanism of recombinant spider silk proteins

Copyright 2008 The National Academy of Sciences of the United States of America ;Copyright National Academy of Sciences May 6, 2008 ;2008 by The National Academy of Sciences of the USA ;ISSN: 0027-8424 ;EISSN: 1091-6490 ;DOI: 10.1073/pnas.0709246105 ;PMID: 18445655

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