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Effect of activated charcoal and ascorbic acid on in vitro morphogenesis and o-dihydroxyphenols content in Paphiopedilum insigne

Horticultural science (Praha), 2022-01, Vol.49 (1), p.48-51 [Peer Reviewed Journal]

2022. This work is published under https://www.agriculturejournals.cz/web/about/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. ;ISSN: 0862-867X ;EISSN: 1805-9333 ;DOI: 10.17221/68/2020-HORTSCI

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  • Title:
    Effect of activated charcoal and ascorbic acid on in vitro morphogenesis and o-dihydroxyphenols content in Paphiopedilum insigne
  • Author: Poniewozik, Monika ; Parzymies, Marzena ; Szot, Paweł
  • Subjects: Activated carbon ; Activated charcoal ; Ascorbic acid ; Charcoal ; lady slipper ; Leaves ; Micropropagation ; Morphogenesis ; Multiplication ; orchid ; Paphiopedilum ; Phenolic compounds ; Phenols ; Rooting ; Shoots ; tissue culture
  • Is Part Of: Horticultural science (Praha), 2022-01, Vol.49 (1), p.48-51
  • Description: Phenolic compounds limit micropropagation of many orchids in vitro. The aim of the study was to estimate the effect of activated charcoal (AC);1, 2 or 4 g/L) or ascorbic acid (AA; 10, 20 or 30 mg/L) added to the half strength MS medium on the growth and o-dihydroxyphenols content in Paphiopedilum insigne in vitro. A positive effect of AC on the shoot and root formation has been found. The highest multiplication rate (5.6 shoots/explant) and rooting frequency were obtained on medium containing 2 g/L of AC. However, AC reduced the leaf number as compared to the control. The lowest content of o-dihydroxyphenols was marked in Paphiopedilum insigne leaves when the shoots were grown on medium with 10 mg/L AA, followed by AC at 1 or 2 g/L.
  • Publisher: Prague: Czech Academy of Agricultural Sciences (CAAS)
  • Language: English;Czech;Slovak
  • Identifier: ISSN: 0862-867X
    EISSN: 1805-9333
    DOI: 10.17221/68/2020-HORTSCI
  • Source: ProQuest Central
    DOAJ Directory of Open Access Journals
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