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A rapid method of species identification of wild chironomids (Diptera: Chironomidae) via electrophoresis of hemoglobin proteins in sodium dodecyl sulfate polyacrylamide gel (SDS–PAGE)

Bulletin of entomological research, 2014-10, Vol.104 (5), p.639-651 [Peer Reviewed Journal]

Copyright © Cambridge University Press 2014 ;2015 INIST-CNRS ;ISSN: 0007-4853 ;ISSN: 1475-2670 ;EISSN: 1475-2670 ;DOI: 10.1017/S0007485314000431 ;PMID: 24923437 ;CODEN: BEREA2

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Title:
A rapid method of species identification of wild chironomids (Diptera: Chironomidae) via electrophoresis of hemoglobin proteins in sodium dodecyl sulfate polyacrylamide gel (SDS–PAGE)
Author: Oh, J.T. ; Epler, J.H. ; Bentivegna, C.S.
Subjects: Animal populations ; Animals ; Biological and medical sciences ; Blood Protein Electrophoresis ; Chironomidae ; Chironomidae - classification ; Chironomus ; Chromatography, Liquid ; Classification - methods ; Cricotopus ; Dicrotendipes ; Diptera ; Electrophoresis, Polyacrylamide Gel ; Fundamental and applied biological sciences. Psychology ; Glyptotendipes ; Hemoglobin ; Hemoglobins - analysis ; Hemoglobins - chemistry ; Insecta ; Insects ; Invertebrates ; Mass Spectrometry ; Morphology ; Research Paper ; Species Specificity ; Systematics. Geographical distribution ; Taxonomy ; Thienemannimyia
Is Part Of: Bulletin of entomological research, 2014-10, Vol.104 (5), p.639-651
Description: Studying aquatic benthic macroinvertebrates (BMIs) in the field requires accurate taxonomic identification, which can be difficult and time consuming. Conventionally, head capsule morphology has been used to identify wild larvae of Chironomidae. However, due to the number of species and possible damage and/or deformity of their head capsules, another supporting approach for identification is needed. Here, we provide hemoglobin (Hb) protein in hemolymph of chironomids as a new biomarker that may help resolve some of the ambiguities and difficulties encountered during taxonomic identification. Chironomids collected from two locations in Maine and New Jersey, USA were identified to the genus level and in some cases to the species-level using head capsule and body morphologies. The head capsule for a particular individual was then associated with a corresponding Hb protein profile generated from sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS–PAGE). Distinct Hb profiles were observed from one group (Thienemannimyia) and four genera (Chironomus, Cricotopus, Dicrotendipes, and Glyptotendipes) of chironomids. Several species were polymorphic, having more than one Hb profile and/or having bands of the same size as those of other species. However, major bands and the combination of bands could distinguish individuals at the genus and sometimes species-level. Overall, this study showed that Hb profiles can be used in combination with head capsule morphology to identify wild chironomids.
Publisher: Cambridge, UK: Cambridge University Press
Language: English
Identifier: ISSN: 0007-4853
ISSN: 1475-2670
EISSN: 1475-2670
DOI: 10.1017/S0007485314000431
PMID: 24923437
CODEN: BEREA2
Source: MEDLINE
ProQuest Central

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A rapid method of species identification of wild chironomids (Diptera: Chironomidae) via electrophoresis of hemoglobin proteins in sodium dodecyl sulfate polyacrylamide gel (SDS–PAGE)

Copyright © Cambridge University Press 2014 ;2015 INIST-CNRS ;ISSN: 0007-4853 ;ISSN: 1475-2670 ;EISSN: 1475-2670 ;DOI: 10.1017/S0007485314000431 ;PMID: 24923437 ;CODEN: BEREA2

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