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The Kubic FLOTAC microscope (KFM): a new compact digital microscope for helminth egg counts

Parasitology, 2021-04, Vol.148 (4), p.427-434 [Peer Reviewed Journal]

Copyright © The Author(s), 2020. Published by Cambridge University Press ;Copyright © The Author(s), 2020. Published by Cambridge University Press. This work is licensed under the Creative Commons Attribution – Non-Commercial – No Derivatives License http://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. ;The Author(s) 2020 2020 The Author(s) ;ISSN: 0031-1820 ;EISSN: 1469-8161 ;DOI: 10.1017/S003118202000219X ;PMID: 33213534

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Title:
The Kubic FLOTAC microscope (KFM): a new compact digital microscope for helminth egg counts
Author: Cringoli, Giuseppe ; Amadesi, Alessandra ; Maurelli, Maria Paola ; Celano, Biase ; Piantadosi, Gabriele ; Bosco, Antonio ; Ciuca, Lavinia ; Cesarelli, Mario ; Bifulco, Paolo ; Montresor, Antonio ; Rinaldi, Laura
Subjects: Artificial intelligence ; Automation ; Cattle ; Correlation coefficient ; Correlation coefficients ; Cost analysis ; Digital cameras ; Digital imaging ; Eggs ; Feces ; Human error ; Intestinal parasites ; Machine learning ; Microscopes ; Nematodes ; Optical microscopes ; Parasites ; Permits ; Public health ; Remote control ; Smartphones ; Software ; Veterinary medicine
Is Part Of: Parasitology, 2021-04, Vol.148 (4), p.427-434
Description: The Kubic FLOTAC microscope (KFM) is a compact, low-cost, versatile and portable digital microscope designed to analyse fecal specimens prepared with Mini-FLOTAC or FLOTAC, in both field and laboratory settings. In this paper, we present the characteristics of the KFM along with its first validation for fecal egg count (FEC) of gastrointestinal nematodes (GINs) in cattle. For this latter purpose, a study was performed on 30 fecal samples from cattle experimentally infected by GINs to compare the performance of Mini-FLOTAC either using a traditional optical microscope (OM) or the KFM. The results of the comparison showed a substantial agreement (concordance correlation coefficient = 0.999), with a very low discrepancy (−0.425 ± 7.370) between the two microscopes. Moreover, the KFM captured images comparable with the view provided by the traditional OM. Therefore, the combination of sensitive, accurate, precise and standardized FEC techniques, as the Mini-FLOTAC, with a reliable automated system, will permit the real-time observation and quantification of parasitic structures, thanks also to artificial intelligence software, that is under development. For these reasons, the KFM is a promising tool for an accurate and efficient FEC to improve parasite diagnosis and to assist new generations of operators in veterinary and public health.
Publisher: Cambridge, UK: Cambridge University Press
Language: English
Identifier: ISSN: 0031-1820
EISSN: 1469-8161
DOI: 10.1017/S003118202000219X
PMID: 33213534
Source: ProQuest Central

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The Kubic FLOTAC microscope (KFM): a new compact digital microscope for helminth egg counts

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