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0.01% Hypochlorous Acid Treats Aspergillus fumigatus Keratitis in Rats by Reducing Fungal Load and Inhibiting the Inflammatory Response

Translational vision science & technology, 2023-08, Vol.12 (8), p.3-3 [Peer Reviewed Journal]

Copyright 2023 The Authors 2023 ;ISSN: 2164-2591 ;EISSN: 2164-2591 ;DOI: 10.1167/tvst.12.8.3 ;PMID: 37531113

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Title:
0.01% Hypochlorous Acid Treats Aspergillus fumigatus Keratitis in Rats by Reducing Fungal Load and Inhibiting the Inflammatory Response
Author: Zhao, Kai ; Hu, Fen ; Zhang, Zhaowei ; Yin, Xiaoyue ; Wang, He ; Li, Mingxin
Subjects: Animals ; Anti-Inflammatory Agents - therapeutic use ; Antifungal Agents - pharmacology ; Antifungal Agents - therapeutic use ; Aspergillosis - drug therapy ; Aspergillosis - metabolism ; Aspergillus fumigatus - physiology ; Cornea & External Disease ; Eye Infections, Fungal - drug therapy ; Eye Infections, Fungal - metabolism ; Eye Infections, Fungal - microbiology ; Hypochlorous Acid - therapeutic use ; Keratitis - drug therapy ; Keratitis - microbiology ; Periodic Acid - therapeutic use ; Peroxidase - therapeutic use ; Rats ; Tumor Necrosis Factor-alpha
Is Part Of: Translational vision science & technology, 2023-08, Vol.12 (8), p.3-3
Description: To investigate the antifungal and anti-inflammatory effects of 0.01% hypochlorous acid (HCLO) on rats with Aspergillus fumigatus keratitis. The time-kill assay and broth microdilution procedures were used in vitro to demonstrate that 0.01% HCLO was fungicidal and fungistatic. The severity of the disease was evaluated in vivo using a clinical score and slit-lamp photographs. Fungal load, polymorphonuclear neutrophil infiltration, and the production of related proteins were determined using colony plate counting, in vivo confocal microscopy, periodic acid-Schiff staining, fungal fluorescence staining, immunofluorescence staining, myeloperoxidase assay, and Western blotting. In vitro, 0.01% HCLO can destroy A. fumigatus spores in 1 minute. The optical density of the 0.01% HCLO group was significantly lower than that of the phosphate-buffered saline control group (P < 0.01), and no visible mycelium was observed using a fluorescence microscope. 0.01% HCLO reduced the severity of A. fumigatus keratitis in rats by decreasing the clinical score, fungal loading (periodic acid-Schiff, plate count, and fungal fluorescence staining), and inhibiting neutrophil infiltration and activity (immunofluorescence staining and myeloperoxidase). Furthermore, the Western blot analysis revealed that 0.01% HCO decreased protein expression levels of tumor necrosis factor-α and IL-1β. According to our findings, 0.01% HCLO can kill A. fumigatus spores in vitro. It has antifungal and anti-inflammatory effects on A. fumigatus keratitis in rats. It also inhibited A. fumigatus growth; decreased neutrophil infiltration, tumor necrosis factor-α, and IL-1β expression; and provided a potential treatment for fungal keratitis. This study provides a potential treatment for fungal keratitis in the clinic.
Publisher: United States: The Association for Research in Vision and Ophthalmology
Language: English
Identifier: ISSN: 2164-2591
EISSN: 2164-2591
DOI: 10.1167/tvst.12.8.3
PMID: 37531113
Source: MEDLINE
PubMed Central
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0.01% Hypochlorous Acid Treats Aspergillus fumigatus Keratitis in Rats by Reducing Fungal Load and Inhibiting the Inflammatory Response

Copyright 2023 The Authors 2023 ;ISSN: 2164-2591 ;EISSN: 2164-2591 ;DOI: 10.1167/tvst.12.8.3 ;PMID: 37531113

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