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Inconsistency of phenotypic and genomic characteristics of Campylobacter fetus subspecies requires reevaluation of current diagnostics

Journal of clinical microbiology, 2014-12, Vol.52 (12), p.4183-4188 [Peer Reviewed Journal]

Copyright © 2014, American Society for Microbiology. All Rights Reserved. ;Copyright © 2014, American Society for Microbiology. All Rights Reserved. 2014 American Society for Microbiology ;Wageningen University & Research ;ISSN: 0095-1137 ;EISSN: 1098-660X ;DOI: 10.1128/jcm.01837-14 ;PMID: 25232170

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Title:
Inconsistency of phenotypic and genomic characteristics of Campylobacter fetus subspecies requires reevaluation of current diagnostics
Author: van der Graaf-van Bloois, Linda ; Miller, William G ; Yee, Emma ; Rijnsburger, Martine ; Wagenaar, Jaap A ; Duim, Birgitta
Munson, E.
Subjects: Animals ; Bacteriological Techniques - methods ; Campylobacter fetus ; Campylobacter fetus - classification ; Campylobacter fetus - genetics ; Campylobacter fetus - isolation & purification ; Campylobacter fetus - physiology ; Campylobacter Infections - diagnosis ; Campylobacter Infections - veterinary ; Cattle ; Cattle Diseases - diagnosis ; Cattle Diseases - microbiology ; Clinical Veterinary Microbiology ; Cluster Analysis ; DNA, Bacterial - genetics ; Genome, Bacterial ; Genotype ; identification ; Molecular Typing ; pcr assay ; Phenotype ; Phylogeny ; time
Is Part Of: Journal of clinical microbiology, 2014-12, Vol.52 (12), p.4183-4188
Description: Classifications of the Campylobacter fetus subspecies fetus and venerealis were first described in 1959 and were based on the source of isolation (intestinal versus genital) and the ability of the strains to proliferate in the genital tract of cows. Two phenotypic assays (1% glycine tolerance and H2S production) were described to differentiate the subspecies. Multiple molecular assays have been applied to differentiate the C. fetus subspecies, but none of these tests is consistent with the phenotypic identification methods. In this study, we defined the core genome and accessory genes of C. fetus, which are based on the closed genomes of five C. fetus strains. Phylogenetic analysis of the core genomes of 23 C. fetus strains of the two subspecies showed a division into two clusters. The phylogenetic core genome clusters were not consistent with the phenotypic classifications of the C. fetus subspecies. However, they were consistent with the molecular characteristics of the strains, which were determined by multilocus sequence typing, sap typing, and the presence/absence of insertion sequences and a type I restriction modification system. The similarity of the genome characteristics of three of the phenotypically defined C. fetus subsp. fetus strains to C. fetus subsp. venerealis strains, when considering the core genome and accessory genes, requires a critical evaluation of the clinical relevance of C. fetus subspecies identification by phenotypic assays.
Publisher: United States: American Society for Microbiology
Language: English
Identifier: ISSN: 0095-1137
EISSN: 1098-660X
DOI: 10.1128/jcm.01837-14
PMID: 25232170
Source: Geneva Foundation Free Medical Journals at publisher websites
MEDLINE
PubMed Central

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Inconsistency of phenotypic and genomic characteristics of Campylobacter fetus subspecies requires reevaluation of current diagnostics

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