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High-fidelity structured illumination microscopy by point-spread-function engineering

Light, science & applications, 2021-04, Vol.10 (1), p.70-70, Article 70 [Peer Reviewed Journal]

The Author(s) 2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. ;The Author(s) 2021 ;ISSN: 2047-7538 ;ISSN: 2095-5545 ;EISSN: 2047-7538 ;DOI: 10.1038/s41377-021-00513-w ;PMID: 33795640

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  • Title:
    High-fidelity structured illumination microscopy by point-spread-function engineering
  • Author: Wen, Gang ; Li, Simin ; Wang, Linbo ; Chen, Xiaohu ; Sun, Zhenglong ; Liang, Yong ; Jin, Xin ; Xing, Yifan ; Jiu, Yaming ; Tang, Yuguo ; Li, Hui
  • Subjects: Fidelity ; Illumination ; Microscopy ; Sectioning
  • Is Part Of: Light, science & applications, 2021-04, Vol.10 (1), p.70-70, Article 70
  • Description: Structured illumination microscopy (SIM) has become a widely used tool for insight into biomedical challenges due to its rapid, long-term, and super-resolution (SR) imaging. However, artifacts that often appear in SIM images have long brought into question its fidelity, and might cause misinterpretation of biological structures. We present HiFi-SIM, a high-fidelity SIM reconstruction algorithm, by engineering the effective point spread function (PSF) into an ideal form. HiFi-SIM can effectively reduce commonly seen artifacts without loss of fine structures and improve the axial sectioning for samples with strong background. In particular, HiFi-SIM is not sensitive to the commonly used PSF and reconstruction parameters; hence, it lowers the requirements for dedicated PSF calibration and complicated parameter adjustment, thus promoting SIM as a daily imaging tool.
  • Publisher: England: Springer Nature B.V
  • Language: English
  • Identifier: ISSN: 2047-7538
    ISSN: 2095-5545
    EISSN: 2047-7538
    DOI: 10.1038/s41377-021-00513-w
    PMID: 33795640
  • Source: Nature Open Access
    PubMed Central
    ProQuest Central
    DOAJ Directory of Open Access Journals
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