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A hevein-like protein and a class I chitinase with antifungal activity from leaves of the paper mulberry

Biomedical chromatography, 2011-08, Vol.25 (8), p.908-912 [Peer Reviewed Journal]

Copyright © 2011 John Wiley & Sons, Ltd. ;ISSN: 0269-3879 ;EISSN: 1099-0801 ;DOI: 10.1002/bmc.1543 ;PMID: 21268047

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Title:
A hevein-like protein and a class I chitinase with antifungal activity from leaves of the paper mulberry
Author: Zhao, Ming ; Ma, Yan ; Pan, Ying-Hong ; Zhang, Chun-Hua ; Yuan, Wen-Xia
Subjects: Amino Acid Sequence ; Antifungal Agents - chemistry ; Antifungal Agents - isolation & purification ; Antifungal Agents - pharmacology ; antifungal protein ; Antimicrobial Cationic Peptides - chemistry ; Antimicrobial Cationic Peptides - isolation & purification ; Antimicrobial Cationic Peptides - physiology ; Chitinases - chemistry ; Chitinases - isolation & purification ; Chitinases - pharmacology ; Chromatography, Ion Exchange ; Drugs, Chinese Herbal ; Electrophoresis, Polyacrylamide Gel ; fast protein liquid chromatography ; matrix-assisted laser desorption ionization time-of-flight mass spectrometry ; Mitosporic Fungi - drug effects ; Molecular Sequence Data ; Molecular Weight ; Morus - chemistry ; Morus - enzymology ; Peptide Mapping ; peptide mapping fingerprint analysis ; Plant Leaves - chemistry ; Plant Lectins - chemistry ; Plant Lectins - isolation & purification ; Plant Lectins - physiology ; Plant Proteins - chemistry ; Plant Proteins - isolation & purification ; Plant Proteins - pharmacology ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Is Part Of: Biomedical chromatography, 2011-08, Vol.25 (8), p.908-912
Description: Paper mulberry (Broussonetia papyrifera, syn. Morus papyrifera L.) is a Chinese traditional medicine and its low‐molecular‐weight extracts are reported to have antifungal activity. In this study, two proteins (PMAPI and PMAPII) with activity against Trichoderma viride were obtained from paper mulberry leaves with a fast protein liquid chromatography (FPLC) unit. The purification protocol employed (NH4)2SO4 precipitation, ion‐exchange chromatography and hydrophobic‐interaction chromatography on FPLC. Molecular masses were 18,798 Da for PMAPI, and 31,178 Da for PMAPII determined by Matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry. Peptide mapping fingerprint analysis showed that PMAPI has no peptides similar to PMAPII. N‐terminal amino acid sequencing revealed that PMAPI is a hevein‐like protein, and PMAPII is a class I chitinase. They both had a half‐maximal inhibitory concentration (IC50) of 0.1 µg/µL against T. viride. This is the first report of high‐molecular‐weight extracts with antifungal activity from paper mulberry. Copyright © 2011 John Wiley & Sons, Ltd.
Publisher: Chichester, UK: John Wiley & Sons, Ltd
Language: English
Identifier: ISSN: 0269-3879
EISSN: 1099-0801
DOI: 10.1002/bmc.1543
PMID: 21268047
Source: MEDLINE
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A hevein-like protein and a class I chitinase with antifungal activity from leaves of the paper mulberry

Copyright © 2011 John Wiley & Sons, Ltd. ;ISSN: 0269-3879 ;EISSN: 1099-0801 ;DOI: 10.1002/bmc.1543 ;PMID: 21268047

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