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Escherichia coli RNA Polymerase Recognition of a σ 70 -Dependent Promoter Requiring a −35 DNA Element and an Extended −10 TGn Motif

Journal of bacteriology, 2006-12, Vol.188 (24), p.8352-8359 [Peer Reviewed Journal]

ISSN: 0021-9193 ;EISSN: 1098-5530 ;DOI: 10.1128/JB.00853-06

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  • Title:
    Escherichia coli RNA Polymerase Recognition of a σ 70 -Dependent Promoter Requiring a −35 DNA Element and an Extended −10 TGn Motif
  • Author: Hook-Barnard, India ; Johnson, Xanthia B. ; Hinton, Deborah M.
  • Is Part Of: Journal of bacteriology, 2006-12, Vol.188 (24), p.8352-8359
  • Description: ABSTRACT Escherichia coli σ 70 -dependent promoters have typically been characterized as either −10/−35 promoters, which have good matches to both the canonical −10 and the −35 sequences or as extended −10 promoters (TGn/−10 promoters), which have the TGn motif and an excellent match to the −10 consensus sequence. We report here an investigation of a promoter, P minor , that has a nearly perfect match to the −35 sequence and has the TGn motif. However, P minor contains an extremely poor σ 70 −10 element. We demonstrate that P minor is active both in vivo and in vitro and that mutations in either the −35 or the TGn motif eliminate its activity. Mutation of the TGn motif can be compensated for by mutations that make the −10 element more canonical, thus converting the −35/TGn promoter to a −35/−10 promoter. Potassium permanganate footprinting on the nontemplate and template strands indicates that when polymerase is in a stable (open) complex with P minor , the DNA is single stranded from positions −11 to +4. We also demonstrate that transcription from P minor incorporates nontemplated ribonucleoside triphosphates at the 5′ end of the P minor transcript, which results in an anomalous assignment for the start site when primer extension analysis is used. P minor represents one of the few −35/TGn promoters that have been characterized and serves as a model for investigating functional differences between these promoters and the better-characterized −10/−35 and extended −10 promoters used by E. coli RNA polymerase.
  • Language: English
  • Identifier: ISSN: 0021-9193
    EISSN: 1098-5530
    DOI: 10.1128/JB.00853-06
  • Source: Geneva Foundation Free Medical Journals at publisher websites
    PubMed Central
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