Language:

Visualization of Oligonucleotide Probes and Point Mutations in Interphase Nuclei and DNA Fibers Using Rolling Circle DNA Amplification

Proceedings of the National Academy of Sciences - PNAS, 2001-03, Vol.98 (7), p.3940-3945 [Peer Reviewed Journal]

Copyright 1993-2001 National Academy of Sciences of the United States of America ;Copyright National Academy of Sciences Mar 27, 2001 ;Copyright © 2001, The National Academy of Sciences 2001 ;ISSN: 0027-8424 ;EISSN: 1091-6490 ;DOI: 10.1073/pnas.061026198 ;PMID: 11274414

Full text available

Citations Cited by

Actions
1. Add to My Research
2. Remove from My Research
3. E-mail
4. Print
5. Permalink
6. Citation
7. EasyBib
8. EndNote
9. RefWorks
10. Delicious
11. Export RIS
12. Export BibTeX

Title:
Visualization of Oligonucleotide Probes and Point Mutations in Interphase Nuclei and DNA Fibers Using Rolling Circle DNA Amplification
Author: Zhong, Xiao-bo ; Lizardi, Paul M. ; Huang, Xiao-hua ; Bray-Ward, Patricia L. ; Ward, David C.
Subjects: Alleles ; Biological Sciences ; BRCA-1 gene ; BRCA1 gene ; Cell Line ; Cell lines ; Cell Nucleus - genetics ; Cystic Fibrosis Transmembrane Conductance Regulator - genetics ; Deoxyribonucleic acid ; DNA ; DNA - analysis ; DNA Mutational Analysis - methods ; DNA probes ; Genes ; Genetic loci ; Genetic mutation ; Genetics ; Gorlin syndrome ; HeLa cells ; Humans ; Interphase ; Interphase - genetics ; Ligation ; Mutation ; Nucleic Acid Amplification Techniques ; Oligonucleotide Probes ; Point Mutation ; Polysorbates ; rolling circle amplification
Is Part Of: Proceedings of the National Academy of Sciences - PNAS, 2001-03, Vol.98 (7), p.3940-3945
Description: Rolling circle amplification (RCA) is a surface-anchored DNA replication reaction that can be exploited to visualize single molecular recognition events. Here we report the use of RCA to visualize target DNA sequences as small as 50 nts in peripheral blood lymphocytes or in stretched DNA fibers. Three unique target sequences within the cystic fibrosis transmembrane conductance regulator gene could be detected simultaneously in interphase nuclei, and could be ordered in a linear map in stretched DNA. Allele-discriminating oligonucleotide probes in conjunction with RCA also were used to discriminate wild-type and mutant alleles in the cystic fibrosis transmembrane conductance regulator, p53, BRCA-1, and Gorlin syndrome genes in the nuclei of cultured cells or in DNA fibers. These observations demonstrate that signal amplification by RCA can be coupled to nucleic acid hybridization and multicolor fluorescence imaging to detect single nucleotide changes in DNA within a cytological context or in single DNA molecules. This provides a means for direct physical haplotyping and the analysis of somatic mutations on a cell-by-cell basis.
Publisher: United States: National Academy of Sciences
Language: English
Identifier: ISSN: 0027-8424
EISSN: 1091-6490
DOI: 10.1073/pnas.061026198
PMID: 11274414
Source: GFMER Free Medical Journals
MEDLINE
PubMed Central

Back to results list


INSPIRE LIBRARY - TON DUC THANG UNIVERSITY	(84-028) 37 755 057	Feedback
19 Nguyen Huu Tho St. Dist.7, HCM	thuvien@tdtu.edu.vn	Feedback

Visualization of Oligonucleotide Probes and Point Mutations in Interphase Nuclei and DNA Fibers Using Rolling Circle DNA Amplification

Copyright 1993-2001 National Academy of Sciences of the United States of America ;Copyright National Academy of Sciences Mar 27, 2001 ;Copyright © 2001, The National Academy of Sciences 2001 ;ISSN: 0027-8424 ;EISSN: 1091-6490 ;DOI: 10.1073/pnas.061026198 ;PMID: 11274414

Searching Remote Databases, Please Wait