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B1 SOX coordinate cell specification with patterning and morphogenesis in the early zebrafish embryo

PLoS genetics, 2010-05, Vol.6 (5), p.e1000936-e1000936 [Peer Reviewed Journal]

Okuda et al. 2010 ;2010 Okuda et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited: Okuda Y, Ogura E, Kondoh H, Kamachi Y (2010) B1 SOX Coordinate Cell Specification with Patterning and Morphogenesis in the Early Zebrafish Embryo. PLoS Genet 6(5): e1000936. doi:10.1371/journal.pgen.1000936 ;ISSN: 1553-7404 ;ISSN: 1553-7390 ;EISSN: 1553-7404 ;DOI: 10.1371/journal.pgen.1000936 ;PMID: 20463883

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Title:
B1 SOX coordinate cell specification with patterning and morphogenesis in the early zebrafish embryo
Author: Okuda, Yuichi ; Ogura, Eri ; Kondoh, Hisato ; Kamachi, Yusuke
van Heyningen, Veronica
Subjects: Animals ; Body Patterning ; Cell adhesion & migration ; Cell Lineage ; Developmental Biology/Cell Differentiation ; Developmental Biology/Developmental Molecular Mechanisms ; Developmental Biology/Morphogenesis and Cell Biology ; Developmental Biology/Neurodevelopment ; Developmental Biology/Pattern Formation ; Developmental Biology/Stem Cells ; Gene expression ; Gene Expression Regulation, Developmental ; Genetics and Genomics/Gene Expression ; Morphogenesis ; Neuroscience/Neurodevelopment ; Proteins ; Regulation ; SOX Transcription Factors - genetics ; SOX Transcription Factors - metabolism ; SOXB1 Transcription Factors - genetics ; SOXB1 Transcription Factors - metabolism ; Studies ; Xenopus Proteins - genetics ; Xenopus Proteins - metabolism ; Zebrafish - embryology ; Zebrafish - genetics ; Zebrafish - growth & development ; Zebrafish - metabolism ; Zebrafish Proteins - genetics ; Zebrafish Proteins - metabolism
Is Part Of: PLoS genetics, 2010-05, Vol.6 (5), p.e1000936-e1000936
Description: The B1 SOX transcription factors SOX1/2/3/19 have been implicated in various processes of early embryogenesis. However, their regulatory functions in stages from the blastula to early neurula remain largely unknown, primarily because loss-of-function studies have not been informative to date. In our present study, we systematically knocked down the B1 sox genes in zebrafish. Only the quadruple knockdown of the four B1 sox genes sox2/3/19a/19b resulted in very severe developmental abnormalities, confirming that the B1 sox genes are functionally redundant. We characterized the sox2/3/19a/19b quadruple knockdown embryos in detail by examining the changes in gene expression through in situ hybridization, RT-PCR, and microarray analyses. Importantly, these phenotypic analyses revealed that the B1 SOX proteins regulate the following distinct processes: (1) early dorsoventral patterning by controlling bmp2b/7; (2) gastrulation movements via the regulation of pcdh18a/18b and wnt11, a non-canonical Wnt ligand gene; (3) neural differentiation by regulating the Hes-class bHLH gene her3 and the proneural-class bHLH genes neurog1 (positively) and ascl1a (negatively), and regional transcription factor genes, e.g., hesx1, zic1, and rx3; and (4) neural patterning by regulating signaling pathway genes, cyp26a1 in RA signaling, oep in Nodal signaling, shh, and mdkb. Chromatin immunoprecipitation analysis of the her3, hesx1, neurog1, pcdh18a, and cyp26a1 genes further suggests a direct regulation of these genes by B1 SOX. We also found an interesting overlap between the early phenotypes of the B1 sox quadruple knockdown embryos and the maternal-zygotic spg embryos that are devoid of pou5f1 activity. These findings indicate that the B1 SOX proteins control a wide range of developmental regulators in the early embryo through partnering in part with Pou5f1 and possibly with other factors, and suggest that the B1 sox functions are central to coordinating cell fate specification with patterning and morphogenetic processes occurring in the early embryo.
Publisher: United States: Public Library of Science
Language: English
Identifier: ISSN: 1553-7404
ISSN: 1553-7390
EISSN: 1553-7404
DOI: 10.1371/journal.pgen.1000936
PMID: 20463883
Source: Open Access: PubMed Central
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B1 SOX coordinate cell specification with patterning and morphogenesis in the early zebrafish embryo

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