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Enzymatic reactions and microorganisms producing the various isomers of hydroxyproline

Applied microbiology and biotechnology, 2020-06, Vol.104 (11), p.4771-4779 [Peer Reviewed Journal]

Springer-Verlag GmbH Germany, part of Springer Nature 2020 ;COPYRIGHT 2020 Springer ;Springer-Verlag GmbH Germany, part of Springer Nature 2020. ;ISSN: 0175-7598 ;EISSN: 1432-0614 ;DOI: 10.1007/s00253-020-10603-1 ;PMID: 32291491

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Title:
Enzymatic reactions and microorganisms producing the various isomers of hydroxyproline
Author: Hara, Ryotaro ; Kino, Kuniki
Subjects: Amino Acid Isomerases - metabolism ; Biocatalysis ; Biological products ; Biomedical and Life Sciences ; Biotechnology ; Crystal structure ; Crystals ; Dioxygenase ; E coli ; Enzyme Induction ; Epimerase ; Escherichia coli ; Escherichia coli - enzymology ; Hydroxylase ; Hydroxylases ; Hydroxylation ; Hydroxyproline ; Hydroxyproline - biosynthesis ; Isomerism ; Isomers ; Ketoglutaric acid ; L-Proline ; Life Sciences ; Metabolism ; Metabolites ; Microbial Genetics and Genomics ; Microbiology ; Microorganisms ; Mini-Review ; Mixed Function Oxygenases - metabolism ; Nutrition ; Physiological aspects ; Proline ; Reviews ; Structure ; Substrates
Is Part Of: Applied microbiology and biotechnology, 2020-06, Vol.104 (11), p.4771-4779
Description: Hydroxyproline is an industrially important compound with applications in the pharmaceutical, nutrition, and cosmetic industries. trans -4-Hydroxy- l -proline is recognized as the most abundant of the eight possible isomers (hydroxy group at C-3 or C-4, cis - or trans -configuration, and l - or d -form). However, little attention has been paid to the rare isomers, probably due to their limited availability. This mini-review provides an overview of recent advances in microbial and enzymatic processes to develop practical production strategies for various hydroxyprolines. Here, we introduce three screening strategies, namely, activity-, sequence-, and metabolite-based approaches, allowing identification of diverse proline-hydroxylating enzymes with different product specificities. All naturally occurring hydroxyproline isomers can be produced by using suitable hydroxylases in a highly regio- and stereo-selective manner. Furthermore, crystal structures of relevant hydroxylases provide much insight into their functional roles. Since hydroxylases acting on free l -proline belong to the 2-oxoglutarate-dependent dioxygenase superfamily, cellular metabolism of Escherichia coli coupled with a hydroxylase is a valuable source of 2-oxoglutarate, which is indispensable as a co-substrate in l -proline hydroxylation. Further, microbial hydroxyproline 2-epimerase may serve as a highly adaptable tool to convert l -hydroxyproline into d -hydroxyproline. Key points • Proline hydroxylases serve as powerful tools for selective l -proline hydroxylation. • Engineered Escherichia coli are a robust platform for hydroxyproline production. • Hydroxyproline epimerase converts l -hydroxyproline into d -hydroxyproline.
Publisher: Berlin/Heidelberg: Springer Berlin Heidelberg
Language: English
Identifier: ISSN: 0175-7598
EISSN: 1432-0614
DOI: 10.1007/s00253-020-10603-1
PMID: 32291491
Source: MEDLINE
ProQuest Central

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Enzymatic reactions and microorganisms producing the various isomers of hydroxyproline

Springer-Verlag GmbH Germany, part of Springer Nature 2020 ;COPYRIGHT 2020 Springer ;Springer-Verlag GmbH Germany, part of Springer Nature 2020. ;ISSN: 0175-7598 ;EISSN: 1432-0614 ;DOI: 10.1007/s00253-020-10603-1 ;PMID: 32291491

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