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SARS‐CoV‐2 Alpha, Beta, and Delta variants display enhanced Spike‐mediated syncytia formation

The EMBO journal, 2021-12, Vol.40 (24), p.e108944-n/a [Tạp chí có phản biện]

2021 The Authors ;2021 The Authors. ;2021 EMBO ;Distributed under a Creative Commons Attribution 4.0 International License ;ISSN: 0261-4189 ;EISSN: 1460-2075 ;DOI: 10.15252/embj.2021108944 ;PMID: 34601723

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Nhan đề:
SARS‐CoV‐2 Alpha, Beta, and Delta variants display enhanced Spike‐mediated syncytia formation
Tác giả: Rajah, Maaran Michael ; Hubert, Mathieu ; Bishop, Elodie ; Saunders, Nell ; Robinot, Remy ; Grzelak, Ludivine ; Planas, Delphine ; Dufloo, Jérémy ; Gellenoncourt, Stacy ; Bongers, Alice ; Zivaljic, Marija ; Planchais, Cyril ; Guivel‐Benhassine, Florence ; Porrot, Françoise ; Mouquet, Hugo ; Chakrabarti, Lisa A ; Buchrieser, Julian ; Schwartz, Olivier
Chủ đề: Abnormalities ; ACE2 ; Affinity ; Angiotensin-Converting Enzyme 2 ; Angiotensin-Converting Enzyme 2 - metabolism ; Animals ; Antibodies, Monoclonal ; Antibodies, Monoclonal - pharmacology ; Binding ; Caco-2 Cells ; Cell fusion ; Cell Line ; Cell surface ; Chlorocebus aethiops ; coronavirus ; COVID-19 ; fusion ; Giant Cells ; Giant Cells - drug effects ; Giant Cells - metabolism ; Giant Cells - virology ; HEK293 Cells ; Humans ; Interferon ; Life Sciences ; Membrane proteins ; Microbiology and Parasitology ; Monoclonal antibodies ; Mutation ; Pneumocytes ; Proteins ; SARS-CoV-2 ; SARS-CoV-2 - drug effects ; SARS-CoV-2 - genetics ; SARS-CoV-2 - physiology ; Severe acute respiratory syndrome ; Severe acute respiratory syndrome coronavirus 2 ; spike ; Spike Glycoprotein, Coronavirus ; Spike Glycoprotein, Coronavirus - genetics ; Spike protein ; Syncytia ; Vero Cells ; Virology ; Virus Replication ; Virus Replication - drug effects
Là 1 phần của: The EMBO journal, 2021-12, Vol.40 (24), p.e108944-n/a
Mô tả: Severe COVID‐19 is characterized by lung abnormalities, including the presence of syncytial pneumocytes. Syncytia form when SARS‐CoV‐2 spike protein expressed on the surface of infected cells interacts with the ACE2 receptor on neighboring cells. The syncytia forming potential of spike variant proteins remain poorly characterized. Here, we first assessed Alpha (B.1.1.7) and Beta (B.1.351) spread and fusion in cell cultures, compared with the ancestral D614G strain. Alpha and Beta replicated similarly to D614G strain in Vero, Caco‐2, Calu‐3, and primary airway cells. However, Alpha and Beta formed larger and more numerous syncytia. Variant spike proteins displayed higher ACE2 affinity compared with D614G. Alpha, Beta, and D614G fusion was similarly inhibited by interferon‐induced transmembrane proteins (IFITMs). Individual mutations present in Alpha and Beta spikes modified fusogenicity, binding to ACE2 or recognition by monoclonal antibodies. We further show that Delta spike also triggers faster fusion relative to D614G. Thus, SARS‐CoV‐2 emerging variants display enhanced syncytia formation. SYNOPSIS The spike protein of the novel SARS‐CoV‐2 variants are comparative more fusogenic than the earlier strains. The mutations in the variant spike protein modulate syncytia formation, ACE2 binding, and antibody escape. The spike protein of Alpha, Beta and Delta, in the absence of other viral proteins, induce more syncytia than the ancestral D614G strain. The ACE2 affinity of the variant spike proteins correlates to their fusogenicity. Variant associated mutations P681H, D1118H, and D215G augment cell‐cell fusion, while antibody escape mutation E484K, K417N and Δ242‐244 hamper it. Spike protein mutations expressed by emerging SARS‐CoV‐2 variants‐of‐concern differentially affect host cell‐to‐cell fusion, ACE2 receptor binding, and antibody escape.
Nơi xuất bản: England: Blackwell Publishing Ltd
Ngôn ngữ: English
Số nhận dạng: ISSN: 0261-4189
EISSN: 1460-2075
DOI: 10.15252/embj.2021108944
PMID: 34601723
Nguồn: GFMER Free Medical Journals
MEDLINE
PubMed Central
Alma/SFX Local Collection

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SARS‐CoV‐2 Alpha, Beta, and Delta variants display enhanced Spike‐mediated syncytia formation

2021 The Authors ;2021 The Authors. ;2021 EMBO ;Distributed under a Creative Commons Attribution 4.0 International License ;ISSN: 0261-4189 ;EISSN: 1460-2075 ;DOI: 10.15252/embj.2021108944 ;PMID: 34601723

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