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Pulp regeneration by transplantation of dental pulp stem cells in pulpitis: a pilot clinical study

Stem cell research & therapy, 2017-03, Vol.8 (1), p.61, Article 61 [Peer Reviewed Journal]

Copyright BioMed Central 2017 ;The Author(s). 2017 ;ISSN: 1757-6512 ;EISSN: 1757-6512 ;DOI: 10.1186/s13287-017-0506-5 ;PMID: 28279187

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Title:
Pulp regeneration by transplantation of dental pulp stem cells in pulpitis: a pilot clinical study
Author: Nakashima, Misako ; Iohara, Koichiro ; Murakami, Masashi ; Nakamura, Hiroshi ; Sato, Yayoi ; Ariji, Yoshiko ; Matsushita, Kenji
Subjects: Adult ; Apoptosis ; Atelocollagen ; Autografts ; Bone marrow ; Cell Differentiation - genetics ; Cell Proliferation - genetics ; Colony-stimulating factor ; Computed tomography ; Dental caries ; Dental pulp ; Dental Pulp - diagnostic imaging ; Dental Pulp - growth & development ; Dental Pulp - pathology ; Dentin ; Endodontics ; Female ; Fractures ; Good Manufacturing Practice ; Granulocyte colony-stimulating factor ; Granulocyte Colony-Stimulating Factor - administration & dosage ; Granulocytes ; Humans ; Leukocytes (granulocytic) ; Magnetic Resonance Imaging ; Male ; Manufacturing ; NMR ; Nuclear magnetic resonance ; Pulpitis - pathology ; Pulpitis - surgery ; Pulpitis - therapy ; Regeneration - genetics ; Root canals ; Spinal cord injuries ; Stem Cell Transplantation ; Stem cells ; Stem Cells - cytology ; Stroke ; Teeth ; Tooth - diagnostic imaging ; Tooth - growth & development ; Tooth - pathology ; Tooth - surgery ; Toxicity ; Transplantation ; Transplants & implants
Is Part Of: Stem cell research & therapy, 2017-03, Vol.8 (1), p.61, Article 61
Description: Experiments have previously demonstrated the therapeutic potential of mobilized dental pulp stem cells (MDPSCs) for complete pulp regeneration. The aim of the present pilot clinical study is to assess the safety, potential efficacy, and feasibility of autologous transplantation of MDPSCs in pulpectomized teeth. Five patients with irreversible pulpitis were enrolled and monitored for up to 24 weeks following MDPSC transplantation. The MDPSCs were isolated from discarded teeth and expanded based on good manufacturing practice (GMP). The quality of the MDPSCs at passages 9 or 10 was ascertained by karyotype analyses. The MDPSCs were transplanted with granulocyte colony-stimulating factor (G-CSF) in atelocollagen into pulpectomized teeth. The clinical and laboratory evaluations demonstrated no adverse events or toxicity. The electric pulp test (EPT) of the pulp at 4 weeks demonstrated a robust positive response. The signal intensity of magnetic resonance imaging (MRI) of the regenerated tissue in the root canal after 24 weeks was similar to that of normal dental pulp in the untreated control. Finally, cone beam computed tomography demonstrated functional dentin formation in three of the five patients. Human MDPSCs are safe and efficacious for complete pulp regeneration in humans in this pilot clinical study.
Publisher: England: BioMed Central
Language: English
Identifier: ISSN: 1757-6512
EISSN: 1757-6512
DOI: 10.1186/s13287-017-0506-5
PMID: 28279187
Source: GFMER Free Medical Journals
MEDLINE
PubMed Central
Springer Nature OA/Free Journals
ProQuest Central
DOAJ Directory of Open Access Journals

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Pulp regeneration by transplantation of dental pulp stem cells in pulpitis: a pilot clinical study

Copyright BioMed Central 2017 ;The Author(s). 2017 ;ISSN: 1757-6512 ;EISSN: 1757-6512 ;DOI: 10.1186/s13287-017-0506-5 ;PMID: 28279187

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