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Enhanced fully cellulose based forward and reverse blood typing assay
info:eu-repo/semantics/openAccess ;ISSN: 1552-4981 ;EISSN: 1552-4981 ;DOI: 10.1002/jbm.b.34400
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Title:
Enhanced fully cellulose based forward and reverse blood typing assay
Author:
Casals Terré, Jasmina
;
Farré Lladós, Josep
;
López Martínez, Joan Antoni
;
Vidal Lluciá, Teresa
;
Roncero Vivero, María Blanca
Subjects:
Blood typing test
;
Cellulose
;
Cel·lulosa
;
Enginyeria paperera
;
Eucalyptus paper
;
Fibres vegetals
;
Investigació
;
Lab-on-a-chip
;
Paper-based microfluidics
;
Papermaking
;
Pasta de paper
;
Plant fibers
;
Point-of-care testing
;
Primeres matèries papereres
;
Sisal (Fiber)
;
Sisal paper
;
Sisal-based paper
;
Tractament
;
Wood-pulp
;
Àrees temàtiques de la UPC
Description:
This study presents an enhanced paper-based analytical device (PAD) for forward and reverse group blood typing. The proposed PAD uses a novel methodology, which provides highly reliable results on a fully cellulose based device. The PAD was printed on different cellulose substrates. These substrates were made of different cellulose fibers (sisal and eucalyptus), different grammages, refining steps, and wet additive content. Best parameters were chosen to achieve high reliability on both forward and reverse blood typing. The substrates were patterned with five hydrophilic channels and two hydrophobic areas. For reverse blood typing, the hemoagglutination reaction took place on the hydrophobic surface of the paper before being transferred to the paper web, where together with the forward blood typing tests were all washed with saline solution to read the results by elution. This device allows direct read-out of results; the stains show were agglutination happens. Different blood types were in full agreement between the reverse and forward method and in agreement with traditional methods. The time and simplicity of this methodology confirmed its utility
Creation Date:
2020-02
Language:
English
Identifier:
ISSN: 1552-4981
EISSN: 1552-4981
DOI: 10.1002/jbm.b.34400
Source:
Recercat
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