Language:

Impacts of silver nanoparticles on cellular and transcriptional activity of nitrogen-cycling bacteria

Environmental toxicology and chemistry, 2013-07, Vol.32 (7), p.1488-1494 [Peer Reviewed Journal]

Copyright © 2013 SETAC ;2014 INIST-CNRS ;Copyright © 2013 SETAC. ;Copyright Blackwell Publishing Ltd. Jul 2013 ;ISSN: 0730-7268 ;EISSN: 1552-8618 ;DOI: 10.1002/etc.2230 ;PMID: 23554086 ;CODEN: ETOCDK

Full text available

Citations Cited by

Actions
1. Add to My Research
2. Remove from My Research
3. E-mail
4. Print
5. Permalink
6. Citation
7. EasyBib
8. EndNote
9. RefWorks
10. Delicious
11. Export RIS
12. Export BibTeX

Title:
Impacts of silver nanoparticles on cellular and transcriptional activity of nitrogen-cycling bacteria
Author: Yang, Yu ; Wang, Jing ; Xiu, Zongming ; Alvarez, Pedro J. J.
Subjects: Aggregation ; Ammonia ; Animal and plant ecology ; Animal, plant and microbial ecology ; Applied ecology ; Bacteria ; Biological and medical sciences ; Denitrification ; Dissolution ; Ecotoxicology, biological effects of pollution ; Fundamental and applied biological sciences. Psychology ; Gene expression ; General aspects ; Genes ; Genes, Bacterial ; Metal Nanoparticles - toxicity ; Nanoparticles ; Nitrogen ; Nitrogen - analysis ; Nitrogen - metabolism ; Nitrogen cycle ; Nitrogen Cycle - drug effects ; Nitrosomonas europaea - drug effects ; Oxidation ; Pseudomonas stutzeri - drug effects ; Pseudomonas stutzeri - genetics ; Pseudomonas stutzeri - metabolism ; Silver - toxicity ; Silver nanoparticle ; Synecology ; Transcription, Genetic - drug effects ; Water Microbiology ; Water Pollutants, Chemical - toxicity ; Water treatment plants
Is Part Of: Environmental toxicology and chemistry, 2013-07, Vol.32 (7), p.1488-1494
Description: The widespread use of silver nanoparticles (AgNPs) raises the potential for environmental releases that could impact microbial ecosystem services. In the present study, the authors address how the AgNPs and Ag+ that they release may impact nitrogen‐cycling bacteria. The authors studied the cellular and transcriptional response of the denitrifier Pseudomonas stutzeri, the nitrogen fixer Azotobacter vinelandii, and the nitrifier Nitrosomonas europaea exposed to 35 nm (carbon‐coated) AgNPs or to Ag+ (added as AgNO3). Based on minimum inhibitory concentrations (MICs), Ag+ was 20 times to 48 times more toxic to the tested strains than AgNPs (including Ag+ released during exposure). Exposure to sublethal concentrations of AgNPs or Ag+ (representing 10% of the respective MIC for AgNO3) resulted in no significant effect on the expression of the denitrifying genes narG, napB, nirH, and norB in P. stutzeri or the nitrogen‐fixing genes nifD, nifH, vnfD, and anfD in A. vinelandii, whereas nitrifying genes (amoA1 and amoC2) in N. europaea were upregulated (2.1‐ to 3.3‐fold). This stimulatory effect disappeared at higher silver concentrations (60% of the Ag+ MIC), and toxicity was exerted at concentrations higher than 60% of the Ag+ MIC. The MIC for N. europaea was 8 times to 24 times lower than for the other strains, indicating higher susceptibility to AgNPs. This was corroborated by the lower half‐lethal concentration for N. europaea (87 µg/L) compared with P. stutzeri (124 µg/L) and A. vinelandii (>250 µg/L) when cells were exposed with Ag+ for 24 h in 1 mM bicarbonate buffer. This suggests that ammonia oxidation would be the most vulnerable nitrogen‐cycling process in wastewater treatment plants receiving AgNPs and in agricultural soils amended with biosolids that concentrate them. Environ Toxicol Chem 2013;32:1488–1494. © 2013 SETAC
Publisher: Pensacola, FL: Blackwell Publishing Ltd
Language: English
Identifier: ISSN: 0730-7268
EISSN: 1552-8618
DOI: 10.1002/etc.2230
PMID: 23554086
CODEN: ETOCDK
Source: MEDLINE
Alma/SFX Local Collection

Back to results list


INSPIRE LIBRARY - TON DUC THANG UNIVERSITY	(84-028) 37 755 057	Feedback
19 Nguyen Huu Tho St. Dist.7, HCM	thuvien@tdtu.edu.vn	Feedback

Impacts of silver nanoparticles on cellular and transcriptional activity of nitrogen-cycling bacteria

Copyright © 2013 SETAC ;2014 INIST-CNRS ;Copyright © 2013 SETAC. ;Copyright Blackwell Publishing Ltd. Jul 2013 ;ISSN: 0730-7268 ;EISSN: 1552-8618 ;DOI: 10.1002/etc.2230 ;PMID: 23554086 ;CODEN: ETOCDK

Searching Remote Databases, Please Wait