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Single-cell transcriptome analysis and in vitro differentiation of testicular cells reveal novel insights into male sterility of the interspecific hybrid cattle-yak

BMC genomics, 2023-03, Vol.24 (1), p.149-149, Article 149 [Peer Reviewed Journal]

2023. The Author(s). ;COPYRIGHT 2023 BioMed Central Ltd. ;2023. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. ;The Author(s) 2023 ;ISSN: 1471-2164 ;EISSN: 1471-2164 ;DOI: 10.1186/s12864-023-09251-2 ;PMID: 36973659

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Title:
Single-cell transcriptome analysis and in vitro differentiation of testicular cells reveal novel insights into male sterility of the interspecific hybrid cattle-yak
Author: Mipam, TserangDonko ; Chen, Xuemei ; Zhao, Wangsheng ; Zhang, Peng ; Chai, Zhixin ; Yue, Binglin ; Luo, Hui ; Wang, Jikun ; Wang, Haibo ; Wu, Zhijuan ; Wang, Jiabo ; Wang, Mingxiu ; Wang, Hui ; Zhang, Ming ; Wang, Hongying ; Jing, Kemin ; Zhong, Jincheng ; Cai, Xin
Subjects: Analysis ; Animal models ; Animals ; Bos grunniens ; Bos taurus ; Care and treatment ; Cattle ; Cattle-yak ; Cell differentiation ; Cell proliferation ; Cells ; Chromosomes ; Developmental stages ; Diagnosis ; Differentiation ; Diseases ; DNA methylation ; Gametocytes ; Gene expression ; Genetic aspects ; Genomics ; Germ cells ; Growth ; Health aspects ; Humans ; Infertility, Male ; Infertility, Male - genetics ; Infertility, Male - veterinary ; Interspecific hybridization ; Male ; Male sterility ; Males ; Meiosis ; Microenvironments ; Niches ; Offspring ; Reproductive isolation ; Single-Cell Gene Expression Analysis ; Single-cell transcriptome ; Sperm ; Spermatogenesis ; Spermatogenesis - genetics ; Spermatogonia ; Testes ; Testicular cells ; Testis - metabolism ; Transcriptomes ; Yaks
Is Part Of: BMC genomics, 2023-03, Vol.24 (1), p.149-149, Article 149
Description: Interspecific hybridization plays vital roles in enriching animal diversity, while male hybrid sterility (MHS) of the offspring commonly suffered from spermatogenic arrest constitutes the postzygotic reproductive isolation. Cattle-yak, the hybrid offspring of cattle (Bos taurus) and yak (Bos grunniens) can serve as an ideal MHS animal model. Although meiotic arrest was found to contribute to MHS of cattle-yak, yet the cellular characteristics and developmental potentials of male germline cell in pubertal cattle-yak remain to be systematically investigated. Single-cell RNA-seq analysis of germline and niche cell types in pubertal testis of cattle-yak and yak indicated that dynamic gene expression of developmental germ cells was terminated at late primary spermatocyte (meiotic arrest) and abnormal components of niche cell in pubertal cattle-yak. Further in vitro proliferation and differentially expressed gene (DEG) analysis of specific type of cells revealed that undifferentiated spermatogonia of cattle-yak exhibited defects in viability and proliferation/differentiation potentials. Comparative scRNA-seq and in vitro proliferation analysis of testicular cells indicated that not only meiotic arrest contributed to MHS of cattle-yak. Spermatogenic arrest of cattle-yak may originate from the differentiation stage of undifferentiated spermatogonia and niche cells of cattle-yak may provide an adverse microenvironment for spermatogenesis.
Publisher: England: BioMed Central Ltd
Language: English
Identifier: ISSN: 1471-2164
EISSN: 1471-2164
DOI: 10.1186/s12864-023-09251-2
PMID: 36973659
Source: Open Access: PubMed Central
Geneva Foundation Free Medical Journals at publisher websites
AUTh Library subscriptions: ProQuest Central
MEDLINE
Springer Open Access Journals
DOAJ: Directory of Open Access Journals
ROAD: Directory of Open Access Scholarly Resources

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Single-cell transcriptome analysis and in vitro differentiation of testicular cells reveal novel insights into male sterility of the interspecific hybrid cattle-yak

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