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0.005% Preservative-Free Latanoprost Induces Dry Eye-Like Ocular Surface Damage via Promotion of Inflammation in Mice

Investigative ophthalmology & visual science, 2018-07, Vol.59 (8), p.3375-3384 [Peer Reviewed Journal]

ISSN: 1552-5783 ;EISSN: 1552-5783 ;DOI: 10.1167/iovs.18-24013 ;PMID: 30025085

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Title:
0.005% Preservative-Free Latanoprost Induces Dry Eye-Like Ocular Surface Damage via Promotion of Inflammation in Mice
Author: Yang, Yiran ; Huang, Caihong ; Lin, Xiang ; Wu, Yang ; Ouyang, Weijie ; Tang, Liying ; Ye, Sihao ; Wang, Yuhong ; Li, Wei ; Zhang, Xiaobo ; Liu, Zuguo
Subjects: Animals ; Antihypertensive Agents - administration & dosage ; Antihypertensive Agents - toxicity ; Blotting, Western ; Conjunctivitis - chemically induced ; Conjunctivitis - metabolism ; Conjunctivitis - pathology ; Cornea - drug effects ; Cornea - metabolism ; Cornea - pathology ; Dry Eye Syndromes - chemically induced ; Dry Eye Syndromes - metabolism ; Dry Eye Syndromes - pathology ; Enzyme-Linked Immunosorbent Assay ; Epithelium, Corneal - physiology ; Female ; Fluorescent Antibody Technique, Indirect ; Humans ; In Situ Nick-End Labeling ; Inflammation - chemically induced ; Inflammation - metabolism ; Inflammation - pathology ; Latanoprost - administration & dosage ; Latanoprost - toxicity ; Matrix Metalloproteinase 3 - genetics ; Matrix Metalloproteinase 3 - metabolism ; Matrix Metalloproteinase 9 - genetics ; Matrix Metalloproteinase 9 - metabolism ; Mice ; Mice, Inbred C57BL ; NF-kappa B - genetics ; NF-kappa B - metabolism ; Occludin - genetics ; Occludin - metabolism ; p38 Mitogen-Activated Protein Kinases - genetics ; p38 Mitogen-Activated Protein Kinases - metabolism ; Preservatives, Pharmaceutical ; Real-Time Polymerase Chain Reaction ; Tears - metabolism ; Zonula Occludens-1 Protein - genetics ; Zonula Occludens-1 Protein - metabolism
Is Part Of: Investigative ophthalmology & visual science, 2018-07, Vol.59 (8), p.3375-3384
Description: To investigate the side effects of preservative-free 0.005% latanoprost on the murine ocular surface. We applied 0.005% latanoprost or vehicle in mice in two patterns for 14 to 28 days. Tear production was measured by phenol red cotton test, and corneal epithelial barrier function was assessed by Oregon-green-dextran (OGD) staining. Periodic acid-Schiff (PAS) staining was used to quantify conjunctival goblet cells (GCs). The expression of matrix metalloproteinase (MMP)-3 and -9, occludin-1 and zonula occludens (ZO)-1 in corneal epithelium was assessed by immunofluorescent staining and/or quantitative real-time PCR (qRT-PCR). Inflammation in conjunctiva was assessed by activation of P38 and NF-κB, infiltration of CD4+ T cells, and production inflammatory cytokines including TNF-α, IL-1β, IFN-γ, IL-17A, and IL-13. Apoptosis in ocular surface was assessed by TUNEL and immunofluorescent staining for activated caspase-3 and -8. Cell viability assay was performed in human corneal epithelial cells. Topical latanoprost treatment decreased tear production, induced conjunctival GC loss, disrupted the corneal epithelial barrier, and promoted cell apoptosis in the ocular surface. Topical latanoprost treatment increased the expression of MMP-3 and -9, and decreased the expression of ZO-1 and occludin-1 in the corneal epithelium. Topical application of latanoprost promoted activation of P38-NF-κB signaling and production of TNF-α and IL-1β in conjunctiva. Topical application of latanoprost increased CD4+ T cells infiltration, with increased production of IFN-γ and IL-17A and decreased production of IL-13 in conjunctiva. 0.005% latanoprost induced dry eye-like ocular surface damage via promotion of inflammation in mice.
Publisher: United States
Language: English
Identifier: ISSN: 1552-5783
EISSN: 1552-5783
DOI: 10.1167/iovs.18-24013
PMID: 30025085
Source: GFMER Free Medical Journals
MEDLINE
PubMed Central
Alma/SFX Local Collection
DOAJ Directory of Open Access Journals

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0.005% Preservative-Free Latanoprost Induces Dry Eye-Like Ocular Surface Damage via Promotion of Inflammation in Mice

ISSN: 1552-5783 ;EISSN: 1552-5783 ;DOI: 10.1167/iovs.18-24013 ;PMID: 30025085

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