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Non-optimal codon usage affects expression, structure and function of clock protein FRQ

Nature (London), 2013-03, Vol.495 (7439), p.111-115 [Peer Reviewed Journal]

COPYRIGHT 2013 Nature Publishing Group ;COPYRIGHT 2013 Nature Publishing Group ;Copyright Nature Publishing Group Mar 7, 2013 ;ISSN: 0028-0836 ;EISSN: 1476-4687 ;DOI: 10.1038/nature11833 ;PMID: 23417067 ;CODEN: NATUAS

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Title:
Non-optimal codon usage affects expression, structure and function of clock protein FRQ
Author: Zhou, Mian ; Guo, Jinhu ; Cha, Joonseok ; Chae, Michael ; Chen, She ; Barral, Jose M ; Sachs, Matthew S ; Liu, Yi
Subjects: Bias ; Circadian Clocks - genetics ; Circadian Clocks - physiology ; Circadian Rhythm - genetics ; Circadian Rhythm - physiology ; CLOCK Proteins - chemistry ; CLOCK Proteins - genetics ; CLOCK Proteins - metabolism ; Codon ; Codon - genetics ; Feedback, Physiological ; Fungal Proteins - chemistry ; Fungal Proteins - genetics ; Fungal Proteins - metabolism ; Gene expression ; Gene Expression Regulation, Fungal ; Genetic aspects ; Genetic transcription ; Genetic translation ; Genetics ; Neurospora ; Neurospora crassa - chemistry ; Neurospora crassa - genetics ; Neurospora crassa - metabolism ; Open Reading Frames ; Phosphorylation ; Properties ; Protein Conformation ; Protein Stability ; Rhythm ; Transfer RNA ; Trypsin - metabolism
Is Part Of: Nature (London), 2013-03, Vol.495 (7439), p.111-115
Description: Codon-usage bias has been observed in almost all genomes and is thought to result from selection for efficient and accurate translation of highly expressed genes. Codon usage is also implicated in the control of transcription, splicing and RNA structure. Many genes exhibit little codon-usage bias, which is thought to reflect a lack of selection for messenger RNA translation. Alternatively, however, non-optimal codon usage may be of biological importance. The rhythmic expression and the proper function of the Neurospora FREQUENCY (FRQ) protein are essential for circadian clock function. Here we show that, unlike most genes in Neurospora, frq exhibits non-optimal codon usage across its entire open reading frame. Optimization of frq codon usage abolishes both overt and molecular circadian rhythms. Codon optimization not only increases FRQ levels but, unexpectedly, also results in conformational changes in FRQ protein, altered FRQ phosphorylation profile and stability, and impaired functions in the circadian feedback loops. These results indicate that non-optimal codon usage of frq is essential for its circadian clock function. Our study provides an example of how non-optimal codon usage functions to regulate protein expression and to achieve optimal protein structure and function.
Publisher: England: Nature Publishing Group
Language: English
Identifier: ISSN: 0028-0836
EISSN: 1476-4687
DOI: 10.1038/nature11833
PMID: 23417067
CODEN: NATUAS
Source: ProQuest One Psychology
AUTh Library subscriptions: ProQuest Central
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Non-optimal codon usage affects expression, structure and function of clock protein FRQ

COPYRIGHT 2013 Nature Publishing Group ;COPYRIGHT 2013 Nature Publishing Group ;Copyright Nature Publishing Group Mar 7, 2013 ;ISSN: 0028-0836 ;EISSN: 1476-4687 ;DOI: 10.1038/nature11833 ;PMID: 23417067 ;CODEN: NATUAS

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