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Preparation and Use of Turn-on Fluorescent Probe for Detection and Live Cell Imaging of Vitamin D Receptor as a Target Protein

STAR protocols, 2020-06, Vol.1 (1), p.100036-100036, Article 100036 [Peer Reviewed Journal]

2020 The Author(s) ;2020 The Author(s). ;2020 The Author(s) 2020 ;ISSN: 2666-1667 ;EISSN: 2666-1667 ;DOI: 10.1016/j.xpro.2020.100036 ;PMID: 33111084

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  • Title:
    Preparation and Use of Turn-on Fluorescent Probe for Detection and Live Cell Imaging of Vitamin D Receptor as a Target Protein
  • Author: Kojima, Hiroyuki ; Itoh, Toshimasa
  • Subjects: Fluorescent Dyes - chemical synthesis ; Fluorescent Dyes - chemistry ; Fluorescent Dyes - metabolism ; HeLa Cells ; Humans ; Molecular Imaging - methods ; Optical Imaging - methods ; Protocol ; Receptors, Calcitriol - analysis ; Receptors, Calcitriol - chemistry ; Receptors, Calcitriol - metabolism
  • Is Part Of: STAR protocols, 2020-06, Vol.1 (1), p.100036-100036, Article 100036
  • Description: Turn-on fluorescent probe mediated by conjugate addition and cyclization (TCC probe) is a small molecule that reacts with a protein of interest in cells. TCC probe is applicable to various types of proteins by exchanging the ligand unit for target proteins. TCC probes are a potent tool for molecular imaging and chemical proteomics. This protocol describes the synthesis of a TCC probe via unstable intermediate and how to use this probe to visualize vitamin D receptor as a target protein. For complete details on the use and execution of this protocol, please refer to Kojima et al. (2020). [Display omitted] •Protocol to prepare a turn-on fluorescent probe for proteins•Method for live cell imaging of a protein of interest•Protocol can be used for chemical proteomics Turn-on fluorescent probe mediated by conjugate addition and cyclization (TCC probe) is a small molecule that reacts with a protein of interest in cells. TCC probe is applicable to various types of proteins by exchanging the ligand unit for target proteins. TCC probes are a potent tool for molecular imaging and chemical proteomics. This protocol describes the synthesis of a TCC probe via unstable intermediate and how to use this probe to visualize vitamin D receptor as a target protein.
  • Publisher: United States: Elsevier Inc
  • Language: English
  • Identifier: ISSN: 2666-1667
    EISSN: 2666-1667
    DOI: 10.1016/j.xpro.2020.100036
    PMID: 33111084
  • Source: MEDLINE
    PubMed Central
    DOAJ Directory of Open Access Journals
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