skip to main content
Language:
Search Limited to: Search Limited to: Resource type Show Results with: Show Results with: Search type Index

Expanding the genetic engineering toolbox for the metabolically flexible acetogen Eubacterium limosum

Journal of industrial microbiology & biotechnology, 2022-07, Vol.49 (5) [Peer Reviewed Journal]

ISSN: 1367-5435 ;EISSN: 1476-5535

Full text available

Citations Cited by
  • Title:
    Expanding the genetic engineering toolbox for the metabolically flexible acetogen Eubacterium limosum
  • Author: Sanford, Patrick A. ; Woolston, Benjamin M.
  • Subjects: Biotechnology & Applied Microbiology
  • Is Part Of: Journal of industrial microbiology & biotechnology, 2022-07, Vol.49 (5)
  • Description: Abstract Acetogenic bacteria are an increasingly popular choice for producing fuels and chemicals from single carbon (C1) substrates. Eubacterium limosum is a promising acetogen with several native advantages, including the ability to catabolize a wide repertoire of C1 feedstocks and the ability to grow well on agar plates. However, despite its promise as a strain for synthetic biology and metabolic engineering, there are insufficient engineering tools and molecular biology knowledge to leverage its native strengths for these applications. To capitalize on the natural advantages of this organism, here we extended its limited engineering toolbox. We evaluated the copy number of three common plasmid origins of replication and devised a method of controlling copy number and heterologous gene expression level by modulating antibiotic concentration. We further quantitatively assessed the strength and regulatory tightness of a panel of promoters, developing a series of well-characterized vectors for gene expression at varying levels. In addition, we developed a black/white colorimetric genetic reporter assay and leveraged the high oxygen tolerance of E. limosum to develop a simple and rapid transformation protocol that enables benchtop transformation. Finally, we developed two new antibiotic selection markers—doubling the number available for this organism. These developments will enable enhanced metabolic engineering and synthetic biology work with E. limosum.
  • Publisher: United States: Springer
  • Language: English
  • Identifier: ISSN: 1367-5435
    EISSN: 1476-5535
  • Source: PubMed Central
    DOAJ Directory of Open Access Journals
Back to results list
INSPIRE LIBRARY - TON DUC THANG UNIVERSITY (84-028) 37 755 057 Feedback
19 Nguyen Huu Tho St. Dist.7, HCM thuvien@tdtu.edu.vn

Copyright © 2017 INSPIRE LIBRARY - TON DUC THANG UNIVERSITY

Searching Remote Databases, Please Wait