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Improved DNA extraction on bamboo paper and cotton is tightly correlated with their crystallinity and hygroscopicity

PloS one, 2022-11, Vol.17 (11), p.e0277138-e0277138 [Tạp chí có phản biện]

COPYRIGHT 2022 Public Library of Science ;2022 Ye, Lei. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. ;2022 Ye, Lei 2022 Ye, Lei ;ISSN: 1932-6203 ;EISSN: 1932-6203 ;DOI: 10.1371/journal.pone.0277138 ;PMID: 36342943

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  • Nhan đề:
    Improved DNA extraction on bamboo paper and cotton is tightly correlated with their crystallinity and hygroscopicity
  • Tác giả: Ye, Xiaojun ; Lei, Bo
  • Fang, David D
  • Chủ đề: Acids ; Adsorbents ; Adsorption ; Analysis ; Bamboo ; Biology and life sciences ; Coronaviruses ; Correlation ; Cotton ; COVID-19 ; COVID-19 diagnostic tests ; Crystallinity ; Decision-making ; Degreasing ; Deoxyribonucleic acid ; DNA ; Efficiency ; Electrophoresis ; Engineering and Technology ; Filter paper ; Health aspects ; High-throughput screening (Biochemical assaying) ; Hygroscopicity ; Innovations ; Medical research ; Methods ; Multidisciplinary research ; Nucleic acids ; Physical Sciences ; Plant species ; Plants (botany) ; Pore size ; Pore size distribution ; Principles ; Research and analysis methods ; Separation ; Size distribution ; Vegetable fibers
  • Là 1 phần của: PloS one, 2022-11, Vol.17 (11), p.e0277138-e0277138
  • Mô tả: DNA extraction, a vital pre-requisite for most biological studies, continues to be studied extensively. According to some studies, DNA shows a certain degree of absorbability on filter paper made of plant fiber-based adsorbent material. However, the principle underlying such specific adsorption as well as plant species associated with plant fiber-based adsorbents and optimized extraction conditions have not yet been studied. This study demonstrates the tight correlation between crystallinity and hygroscopicity in plant fiber-based adsorbents used for DNA extraction and proposes the concept of DNA adsorption on plant fiber-based adsorbents, for the first time. We also explored optimal extracting and eluting conditions and developed a novel plant fiber-based DNA extraction method that was quadruple times more powerful than current approaches. Starting with the screening of various types of earthed plant fiber-based adsorbents, we went on to mine new plant fiber-based adsorbents, bamboo paper and degreased cotton, and succeeded in increasing their efficiency of DNA extraction to 4.2 times than that of current approaches. We found a very strong correlation between the crystallinity and hygroscopicity of plant fiber-based adsorbents which showed efficiency for DNA extraction, and thus propose a principle that potentially governs such specific adsorption processes, in the hope that this information may guide related multidisciplinary research studies in the future. Nanodrop, electrophoresis and PCR were selected to demonstrate the quantity, quality, integrity and utility of the extracted DNA. Furthermore, crystallinity, hygroscopicity, pore size distribution and composition of plant fiber-based adsorbents were studied to explore their correlation in an attempt to understand the principle underlying this particular type of adsorption. The findings of this study may be further extended to the extraction of other types of nucleic acids with similar biochemical properties.
  • Nơi xuất bản: San Francisco: Public Library of Science
  • Ngôn ngữ: English
  • Số nhận dạng: ISSN: 1932-6203
    EISSN: 1932-6203
    DOI: 10.1371/journal.pone.0277138
    PMID: 36342943
  • Nguồn: Public Library of Science (PLoS) Journals Open Access
    Freely Accessible Journals
    AUTh Library subscriptions: ProQuest Central
    PubMed Central
    Coronavirus Research Database
    DOAJ Directory of Open Access Journals
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