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BAS/BSCR38 Cytokine profiling in culture reveals a predominance of M1 macrophage polarisation in symptomatic carotid plaques

Heart (British Cardiac Society), 2010-09, Vol.96 (17), p.e23-e23 [Peer Reviewed Journal]

2010, Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions. ;Copyright: 2010 (c) 2010, Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions. ;ISSN: 1355-6037 ;ISSN: 1468-201X ;EISSN: 1468-201X ;DOI: 10.1136/hrt.2010.205781.49

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  • Title:
    BAS/BSCR38 Cytokine profiling in culture reveals a predominance of M1 macrophage polarisation in symptomatic carotid plaques
  • Author: Shalhoub, J ; Cross, A ; Allin, D ; Essex, D ; Davies, A H ; Monaco, C
  • Subjects: Arteriosclerosis
  • Is Part Of: Heart (British Cardiac Society), 2010-09, Vol.96 (17), p.e23-e23
  • Description: RationaleMacrophages in atherosclerotic plaques are a heterogeneous population. To uncover signatures of classical (M1) or alternative (M2) macrophage polarisation during plaque instability, we compared cytokine and chemokine production via Luminex profiling in asymptomatic human carotid plaques with carotid plaques in the territory of recent focal neurological symptomatology.MethodologyCarotid endarterectomy specimens were collected from 50 consenting patients (26 symptomatic, 24 asymptomatic). Fresh specimens were divided symmetrically along their long axis, allowing for representative undertaking of both immunohistochemistry and plaque cell culture (Monaco et al. PNAS USA 2004;101:5634–9). Automated image analysis (Clemex Vision) quantified CD68 staining. Cells were isolated via enzymatic dissociation to produce a viable mixed cell suspension and cultured for 24 h. Macrophage was the predominant cell type in the mixed cell culture. Supernatants were interrogated with a panel of 22 cytokines and chemokines on a Luminex 100 platform.ResultsCD68 immunopositivity was significantly higher in symptomatic than in asymptomatic plaques (p=0.0075). Luminex detected 17 of the 22 analytes, with a predominance of myeloid-derived cytokines over lymphoid-derived cytokines, in keeping with the predominance of macrophage in culture. Tumour necrosis factor α, interleukin (IL)-1α, IL-1β, IL-6, granulocyte-macrophage colony-stimulating factor, CCL2, CCL5 and IL-10 levels were significantly higher in symptomatic plaques.ConclusionsOur data demonstrate that symptomatic atherosclerotic carotid disease is associated with a cytokine pattern consistent with the predominance of proinflammatory M1-type macrophage polarisation. M2-dependent cytokine IL-10 was also part of this inflammatory response, suggesting macrophage heterogeneity. Our study has implications for future therapeutic and diagnostic applications for high-risk atherosclerosis.
  • Publisher: London: BMJ Publishing Group Ltd and British Cardiovascular Society
  • Language: English
  • Identifier: ISSN: 1355-6037
    ISSN: 1468-201X
    EISSN: 1468-201X
    DOI: 10.1136/hrt.2010.205781.49
  • Source: Alma/SFX Local Collection
    ProQuest Central
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