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Long Noncoding RNA MALAT1 Regulates Hepatocellular Carcinoma Growth Under Hypoxia via Sponging MicroRNA-200a

Yonsei Medical Journal, 2019, 60(8), , pp.727-734 [Peer Reviewed Journal]

COPYRIGHT(C) KYOBO BOOK CENTRE ALL RIGHTS RESERVED ;Copyright: Yonsei University College of Medicine 2019. ;Copyright: Yonsei University College of Medicine 2019 2019 Yonsei University College of Medicine ;ISSN: 0513-5796 ;EISSN: 1976-2437 ;DOI: 10.3349/ymj.2019.60.8.727 ;PMID: 31347327

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  • Title:
    Long Noncoding RNA MALAT1 Regulates Hepatocellular Carcinoma Growth Under Hypoxia via Sponging MicroRNA-200a
  • Author: Zhao, Zheng Bin ; Chen, Fei ; Bai, Xiao Fang
  • Subjects: Original ; 의학일반
  • Is Part Of: Yonsei Medical Journal, 2019, 60(8), , pp.727-734
  • Description: Hepatocellular carcinoma (HCC) is a common cancer worldwide. Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), a long noncoding RNA (lncRNA), has been reported to be aberrantly expressed in hypoxic cancer cells. MALAT1 plays a significant role in many malignancies, including HCC. The aim of this study was to explore the role of MALAT1 in hypoxic HCC cells and its underlying regulatory mechanism. Quantitative reverse transcription PCR (qRT-PCR) assay was performed to detect the mRNA levels of MALAT1 and microRNA-200a (miR-200a) in HCC cells. Cell invasion and migration ability were evaluated by Transwell assay. Starbase v2.0 and luciferase reporter assay were employed to identify the association between MALAT1 and miR-200a. Cell proliferation and apoptosis were measured by MTT assay and flow cytometry, respectively. MALAT1 levels were significantly upregulated in HCC cells under hypoxia. Hypoxia promoted proliferation, migration, and invasion, and blocked apoptosis in Hep3B cells, which were weakened by knockdown of MALAT1. Starbase v2.0 showed that MALAT1 and miR-200a have a complementarity region, and luciferase reporter assay verified that MALAT1 interacted with miR-200a in Hep3B cells. Moreover, MALAT1 negatively regulated the expression of miR-200a. miR-200a levels were dramatically downregulated in HCC cells under hypoxia. Upregulation of miR-200a inhibited proliferation, migration, and invasion, and induced apoptosis in Hep3B cells under hypoxia. Interestingly, downregulation of miR-200a partially reversed the tumor-suppressive effect of knockdown of MALAT1 on Hep3B cells in hypoxic condition. LncRNA MALAT1 was involved in proliferation, migration, invasion, and apoptosis by interacting with miR-200a in hypoxic Hep3B cells, revealing a new mechanism of MALAT1 involved in hypoxic HCC progression.
  • Publisher: Korea (South): 연세대학교의과대학
  • Language: Korean;English
  • Identifier: ISSN: 0513-5796
    EISSN: 1976-2437
    DOI: 10.3349/ymj.2019.60.8.727
    PMID: 31347327
  • Source: PubMed (Medline)
    KoreaMed Synapse(OpenAccess)
    Geneva Foundation Free Medical Journals at publisher websites
    KoreaMed Open Access
    ROAD: Directory of Open Access Scholarly Resources
    DOAJ Directory of Open Access Journals
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