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method for isolation of milled-wood lignin involving solvent swelling prior to enzyme treatment

Wood science and technology, 1995-06, Vol.29 (4), p.295-306 [Peer Reviewed Journal]

1995 INIST-CNRS ;ISSN: 0043-7719 ;EISSN: 1432-5225 ;DOI: 10.1007/BF00202089 ;CODEN: WOSTBE

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  • Title:
    method for isolation of milled-wood lignin involving solvent swelling prior to enzyme treatment
  • Author: Chen, J.Y ; Shimizu, Y ; Takai, M ; Hayashi, J
  • Subjects: Applied sciences ; cellulases ; Exact sciences and technology ; isolation ; lignin ; Lignin and derivatives ; Natural polymers ; Physicochemistry of polymers ; solvents ; swelled enzyme lignin ; wood ; wood pulp
  • Is Part Of: Wood science and technology, 1995-06, Vol.29 (4), p.295-306
  • Description: A new lignin isolation method has been developed. Wood and pulp were subjected to ball milling, swelled in an organic solvent, and then treated with a cellulase. The enzyme digestion time could be shortened to 1 day with this method. The lignin obtained has been named Swelled Enzyme Lignin (SEL). Swelling and enzyme digestion conditions and their effects on lignins were investigated. The SEL's from wood could be directly washed with water, while those from pulp had to be washed with aqueous acetic acid because they were water soluble. The purification of crude SEL's was accomplished by extracting them with dioxane-water, and then precipitating and washing with ethyl ether. Lignin yields were 24-67% based on the total amount of lignin present. The characteristics of the SEL's were further investigated by gel-permeation chromatography (GPC), infrared and 13C nuclear magnetic resonance (NMR) spectroscopy.
  • Publisher: Berlin: Springer
  • Language: English
  • Identifier: ISSN: 0043-7719
    EISSN: 1432-5225
    DOI: 10.1007/BF00202089
    CODEN: WOSTBE
  • Source: Alma/SFX Local Collection
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