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A novel protein encoded by circMAPK1 inhibits progression of gastric cancer by suppressing activation of MAPK signaling

Molecular cancer, 2021-04, Vol.20 (1), p.66-66, Article 66 [Peer Reviewed Journal]

COPYRIGHT 2021 BioMed Central Ltd. ;2021. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. ;The Author(s) 2021 ;ISSN: 1476-4598 ;EISSN: 1476-4598 ;DOI: 10.1186/s12943-021-01358-y ;PMID: 33836754

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Title:
A novel protein encoded by circMAPK1 inhibits progression of gastric cancer by suppressing activation of MAPK signaling
Author: Jiang, Tianlu ; Xia, Yiwen ; Lv, Jialun ; Li, Bowen ; Li, Ying ; Wang, Sen ; Xuan, Zhe ; Xie, Li ; Qiu, Shengkui ; He, Zhongyuan ; Wang, Linjun ; Xu, Zekuan
Subjects: Analysis ; Antisense RNA ; Apoptosis ; Bioinformatics ; Cancer ; Cell growth ; Cell proliferation ; Cellular signal transduction ; Circular RNA ; Coding capacity ; Development and progression ; Gastric cancer ; Gene expression ; Immunofluorescence ; Immunoprecipitation ; Investigations ; Kinases ; MAP kinase ; MAPK pathway ; Mass spectrometry ; Mass spectroscopy ; Medical prognosis ; Medical research ; Medicine, Experimental ; Metastasis ; MicroRNAs ; Phosphorylation ; Physiology ; Proteins ; Signal transduction ; Stomach cancer ; Therapeutic targets ; Tumor suppressor genes ; Tumorigenesis
Is Part Of: Molecular cancer, 2021-04, Vol.20 (1), p.66-66, Article 66
Description: A novel type of noncoding RNA, circRNA has been reported to participate in the occurrence and development of diseases through many mechanisms. The MAPK pathway is a common signal transduction pathway involved in cell proliferation, inflammation and apoptosis and plays a particularly important role in cancers. However, the role of circRNAs related to the MAPK pathway in gastric cancer has not been explored. A bioinformatics analysis was performed to profile and identify the circRNAs involved in the MAPK pathway in gastric cancer. The tumor-suppressive role of circMAPK1 was confirmed both in vitro and in vivo. Mass spectrometry, Western blot and immunofluorescence staining assays were used to validate the existence and expression of MAPK1-109aa. The molecular mechanism of circMAPK1 was investigated by mass spectrometry and immunoprecipitation analyses. In this study, we identified that circMAPK1 (hsa_circ_0004872) was downregulated in gastric cancer tissues compared with adjacent normal tissues. Importantly, lower circMAPK1 expression predicted poor survival in GC patients. CircMAPK1 inhibited the proliferation and invasion of gastric cancer cells in vitro and in vivo. Next, we found that circMAPK1 encoded a novel protein with 109 amino acids in length. Through a series of functional experiments, we confirmed that circMAPK1 exerted a tumor-suppressing effect via the encoded protein MAPK1-109aa. Mechanistically, the tumor suppressor MAPK1-109aa inhibited the phosphorylation of MAPK1 by competitively binding to MEK1, thereby suppressing the activation of MAPK1 and its downstream factors in MAPK pathway. Our study revealed that circMAPK1 inhibits the malignant biological behavior of gastric cancer cells through its encoded protein MAPK1-109aa. More importantly, circMAPK1 is a favorable predictor for gastric cancer patients and may provide a new therapeutic target in the treatment of gastric cancer.
Publisher: England: BioMed Central Ltd
Language: English
Identifier: ISSN: 1476-4598
EISSN: 1476-4598
DOI: 10.1186/s12943-021-01358-y
PMID: 33836754
Source: GFMER Free Medical Journals
PubMed Central
Springer Nature OA/Free Journals
ROAD: Directory of Open Access Scholarly Resources
ProQuest Central
DOAJ Directory of Open Access Journals

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A novel protein encoded by circMAPK1 inhibits progression of gastric cancer by suppressing activation of MAPK signaling

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