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Core packing of well‐defined X‐ray and NMR structures is the same
Protein science, 2022-08, Vol.31 (8), p.e4373-n/a
[Peer Reviewed Journal]
2022 The Protein Society. ;2022 The Protein Society ;ISSN: 0961-8368 ;EISSN: 1469-896X ;DOI: 10.1002/pro.4373 ;PMID: 35900019
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Title:
Core packing of well‐defined X‐ray and NMR structures is the same
Author:
Grigas, Alex T.
;
Liu, Zhuoyi
;
Regan, Lynne
;
O'Hern, Corey S.
Subjects:
Chemical bonds
;
Constraints
;
Cores
;
Crystal structure
;
Crystallography
;
Crystallography, X-Ray
;
Crystals
;
Dihedral angle
;
Full‐length Paper
;
Full‐length Papers
;
hydrophobic core
;
Hydrophobicity
;
Magnetic resonance spectroscopy
;
Magnetic Resonance Spectroscopy - methods
;
NMR
;
NMR spectroscopy
;
Nuclear magnetic resonance
;
Nuclear Magnetic Resonance, Biomolecular - methods
;
Outliers (statistics)
;
Packing
;
Protein Conformation
;
protein design
;
protein structure
;
Proteins
;
Proteins - chemistry
;
Stereochemistry
;
X-Rays
Is Part Of:
Protein science, 2022-08, Vol.31 (8), p.e4373-n/a
Description:
Numerous studies have investigated the differences and similarities between protein structures determined by solution NMR spectroscopy and those determined by X‐ray crystallography. A fundamental question is whether any observed differences are due to differing methodologies or to differences in the behavior of proteins in solution versus in the crystalline state. Here, we compare the properties of the hydrophobic cores of high‐resolution protein crystal structures and those in NMR structures, determined using increasing numbers and types of restraints. Prior studies have reported that many NMR structures have denser cores compared with those of high‐resolution X‐ray crystal structures. Our current work investigates this result in more detail and finds that these NMR structures tend to violate basic features of protein stereochemistry, such as small non‐bonded atomic overlaps and few Ramachandran and sidechain dihedral angle outliers. We find that NMR structures solved with more restraints, and which do not significantly violate stereochemistry, have hydrophobic cores that have a similar size and packing fraction as their counterparts determined by X‐ray crystallography at high resolution. These results lead us to conclude that, at least regarding the core packing properties, high‐quality structures determined by NMR and X‐ray crystallography are the same, and the differences reported earlier are most likely a consequence of methodology, rather than fundamental differences between the protein in the two different environments.
Publisher:
Hoboken, USA: John Wiley & Sons, Inc
Language:
English
Identifier:
ISSN: 0961-8368
EISSN: 1469-896X
DOI: 10.1002/pro.4373
PMID: 35900019
Source:
Open Access: PubMed Central
Geneva Foundation Free Medical Journals at publisher websites
MEDLINE
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